Czerwinska Krystyna, Wyszomirska Elzbieta, Kublin Elzbieta, Malanowic Ewa, Mazurek Aleksander P
Acta Pol Pharm. 2016 Nov;73(6):1467-1474.
Simple, precise and accurate densitometric methods were developed for the determination of two antihistamine drugs. rupatadine and fexofenadine. Silica gel 60 F₂₅₄ HPTLC plates were used as stationary phase, while mixtures of acetonitrile - water - 25% ammonia (90 : 10 : 1, v/v/v) and acetonitrile - methanol -acetate buffer at pH 5.5 (3 : 2 : 5, v/v/v) were used as mobile phases for rupatadine and fexofenadine, respectively. The detection of rupatadine and fexofenadine was conducted out at 256 and 210 nm, respectively. The limit of detection and the limit of quantification for rupatadine were found to be 0.3 and 0.1 μg/spot, respectively, and for fexofenadine, 5 and 2 μg/spot, respectively.
