Bilska-Wilkosz Anna, Gorny Magdalena, Dudek Magdalena, Nowinski Leszek, Bednarski Marek, Iciek Malgorzata, Kowalczyk-Pachel Danuta, Sokolowska-Jezewicz Maria, Filipek Barbara, Wlodek Lidia
Acta Pol Pharm. 2016 Nov;73(6):1531-1538.
Lipoic acid (LA-(SS), LA) and its reduced form - dihydrolipoic acid DHLA-(SH)2, DHLA) are synthesized mainly in the mammalian liver. In this study, we investigated in viti the inactivation of yeast aldehyde dehydrogenase (ALDH) by nitroglycerin (GTN) in the presence and absence of LA and DHLA. In vivo studies were performed to answer the question whether LA administered jointly with GTN for 8 days will affect the ALDH activity in the rat liver. The results indicated that in vito both LA and DHLA restored and protected ALDH activity against GTN-induced inactivation, while treatment of rats with LA and GTN in combination did not provide any protection against GTN-induced ALDH inhibition. In summary, the obtained results seem to confirm earlier reports indicating the differential effects of LA in vitio and in vivo.
硫辛酸（LA-(SS)，LA）及其还原形式——二氢硫辛酸（DHLA-(SH)2，DHLA）主要在哺乳动物肝脏中合成。在本研究中，我们研究了在有和没有LA及DHLA存在的情况下，硝酸甘油（GTN）对酵母醛脱氢酶（ALDH）的体外失活作用。进行了体内研究以回答与GTN联合给药8天的LA是否会影响大鼠肝脏中ALDH活性这一问题。结果表明，在体外，LA和DHLA均可恢复并保护ALDH活性免受GTN诱导的失活影响，而用LA和GTN联合处理大鼠并未对GTN诱导的ALDH抑制提供任何保护。总之，所得结果似乎证实了早期报告，表明LA在体外和体内具有不同的作用。
