Imanishi J, Watanabe H, Horii Y, Sugimoto T
Département de Microbiologie, Université Médicale de la Préfecture de Kyoto.
C R Seances Soc Biol Fil. 1987;181(4):454-7.
In the previous study, it was shown that the treatment of human neuroblastoma cells with human interferon-gamma (HuIFN-gamma) induced the morphological changes. However, the treatment with human interferon-alpha (HuIFN-alpha) or -beta (HuIFN-beta) did not induce them. In the present study, the effect of HuIFNs on the overexpression of N-myc of the human neuroblastoma cells (GOTO strain) is examined. The treatment of GOTO cells with rHuIFN-gamma inhibits the overexpression of N-myc, and its degree is dependent on the duration of the treatment. However, HuIFN-alpha and HuIFN-beta did not inhibit the overexpression of N-myc. This suggests that the oncogene N-myc may have relation to the morphological differentiation of human neuroblastoma cells because only the HuIFN-gamma, which induces the morphological differentiation, inhibits the overexpression of N-myc.
在先前的研究中，已表明用人γ干扰素（HuIFN-γ）处理人神经母细胞瘤细胞可诱导形态学变化。然而，用人α干扰素（HuIFN-α）或β干扰素（HuIFN-β）处理则不会诱导这种变化。在本研究中，检测了HuIFN对人神经母细胞瘤细胞（GOTO株）N-myc过表达的影响。用重组人γ干扰素（rHuIFN-γ）处理GOTO细胞可抑制N-myc的过表达，其抑制程度取决于处理持续时间。然而，HuIFN-α和HuIFN-β并未抑制N-myc的过表达。这表明癌基因N-myc可能与人神经母细胞瘤细胞的形态分化有关，因为只有诱导形态分化的HuIFN-γ能抑制N-myc的过表达。
