代谢途径的动力学。磷酸果糖激酶反应后糖酵解系统对ADP输入的瞬态响应。

Kinetics of metabolic pathways. Transient response of the glycolytic system after phosphofructokinase reaction to ADP input.

Riol-Cimas J M, Meléndez-Hevia E

Departamento de Bioquímica y Biología Molecular, Facultad de Biología, Universidad de La Laguan, Canary Islands, Spain.

Int J Biochem. 1988;20(1):29-33. doi: 10.1016/0020-711x(88)90006-7.

Abstract

Transient response of the glycolytic system, after phosphofructokinase reaction, to ADP input was studied in a metabolic model in vitro, obtained from rat muscle. 2. Under ADP addition to the system, NADH concentration is largely enhanced to be decreased later, showing a very characteristic pulse whose parameters can be used to describe some features of the transition state. 3. NADH pulse was repeatedly obtained by means of successive ADP inputs to the system. 4. The effect of different variables on NADH pulse was studied, these results suggesting a possible role for different steps on this response. 5. The possible relation between the pulse of NADH and oscillatory behaviour of glycolysis is discussed.

摘要

在体外从大鼠肌肉获得的代谢模型中，研究了磷酸果糖激酶反应后糖酵解系统对ADP输入的瞬态响应。2. 向系统中添加ADP后，NADH浓度大幅升高，随后降低，呈现出非常典型的脉冲，其参数可用于描述过渡态的某些特征。3. 通过向系统连续输入ADP可反复获得NADH脉冲。4. 研究了不同变量对NADH脉冲的影响，这些结果表明不同步骤在该响应中可能发挥作用。5. 讨论了NADH脉冲与糖酵解振荡行为之间的可能关系。