无白蛋白血症大鼠肝脏中转铁蛋白mRNA水平较高。

A high level of transferrin mRNA in the liver of analbuminemic rats.

作者信息

Sugiyama K, Izumi S, Tomino S, Nagase S

机构信息

Department of Chemistry, Sasaki Institute, Tokyo.

出版信息

J Biochem. 1987 Nov;102(5):967-70. doi: 10.1093/oxfordjournals.jbchem.a122172.

DOI:10.1093/oxfordjournals.jbchem.a122172

PMID:2963811

Abstract

By means of immunological screening, a cDNA clone bearing the mRNA sequence for rat transferrin was isolated from a cDNA library of rat liver mRNA. The amounts of transferrin mRNA in livers of analbuminemic rats (NAR, Nagase analbuminemia rats) and normal rats were determined by RNA blot hybridization using a cloned transferrin cDNA probe. The level of transferrin mRNA in the NAR liver was about 1.7 times that in the normal rat liver. These findings suggest that the enhanced synthesis of transferrin in the NAR liver resulted from an increase in the transferrin mRNA level.

摘要

通过免疫筛选，从大鼠肝脏mRNA的cDNA文库中分离出一个携带大鼠转铁蛋白mRNA序列的cDNA克隆。使用克隆的转铁蛋白cDNA探针，通过RNA印迹杂交法测定了无白蛋白血症大鼠（NAR，长濑无白蛋白血症大鼠）和正常大鼠肝脏中转铁蛋白mRNA的含量。NAR肝脏中转铁蛋白mRNA的水平约为正常大鼠肝脏的1.7倍。这些发现表明，NAR肝脏中转铁蛋白合成的增强是由于转铁蛋白mRNA水平的增加所致。