Grant D S, Leblond C P
Department of Anatomy, McGill University, Montreal, Quebec, Canada.
J Histochem Cytochem. 1988 Mar;36(3):271-83. doi: 10.1177/36.3.2963856.
A series of basement membranes was immunolabeled for laminin, type IV collagen, and heparan sulfate proteoglycan in the hope of comparing the content of these substances. The basement membranes, including thin ones (less than 0.3 micron) from kidney, colon, enamel organ, and vas deferens, and thick ones (greater than 2 micron), i.e., Reichert's membrane, Descemet's membrane, and EHS tumor matrix, were fixed in formaldehyde, embedded in Lowicryl, and treated with specific antisera or antibodies followed by anti-rabbit immunoglobulin bound to gold. The density of gold particles, expressed per micron2, was negligible in controls (less than or equal to 1.1), but averaged 307, 146, and 23, respectively, for laminin, collagen IV, and proteoglycan over the thick basement membranes (except for Descemet's membranes, over which the density was 16, 5, and 34, respectively) and 117, 72, and 64, respectively, over the lamina densa of the thin basement membranes. Lower but significant reactions were observed over the lamina lucida. Interpretation of the gold particle densities was based on (a) the similarity between the ultrastructure of most thick basement membranes and of the lamina densa of most thin basement membranes, and (b) the biochemical content of the three substances under study in the EHS tumor matrix (Eur J Biochem 143:145, 1984). It was proposed that thick basement membranes (except Descemet's) contained more laminin and collagen IV but less heparan sulfate proteoglycan than the lamina densa of thin basement membranes. In the latter, there was a fair variation from tissue to tissue, but a tendency towards a similar molar content of the three substances.
为了比较这些物质的含量，对一系列基底膜进行了层粘连蛋白、IV型胶原和硫酸乙酰肝素蛋白聚糖的免疫标记。基底膜包括来自肾脏、结肠、釉质器和输精管的薄基底膜（小于0.3微米）以及厚基底膜（大于2微米），即瑞氏膜、Descemet膜和EHS肿瘤基质，将其用甲醛固定，包埋在Lowicryl中，并用特异性抗血清或抗体处理，随后用与金结合的抗兔免疫球蛋白处理。以每平方微米表示的金颗粒密度在对照中可忽略不计（小于或等于1.1），但在厚基底膜上（Descemet膜除外，其密度分别为16、5和34），层粘连蛋白、IV型胶原和蛋白聚糖的平均密度分别为307、146和23，在薄基底膜的致密层上分别为117、72和64。在透明层上观察到较低但显著的反应。金颗粒密度的解释基于：（a）大多数厚基底膜的超微结构与大多数薄基底膜的致密层之间的相似性，以及（b）EHS肿瘤基质中所研究的三种物质的生化含量（《欧洲生物化学杂志》143:145，1984）。有人提出，厚基底膜（Descemet膜除外）比薄基底膜的致密层含有更多的层粘连蛋白和IV型胶原，但硫酸乙酰肝素蛋白聚糖较少。在薄基底膜中，不同组织之间存在相当大的差异，但三种物质的摩尔含量有相似的趋势。
