Bucy R P, Hanto D W, Berens E, Schreiber R D
Department of Pathology, Washington University School of Medicine, St. Louis, MO 63110.
J Immunol. 1988 Feb 15;140(4):1148-52.
We have investigated the relative importance of two lymphokines, IL-2 and IFN-gamma, in the primary murine MLR. Three separate lines of evidence indicate that IL-2 and not IFN-gamma is the relevant lymphokine for both proliferation and activation of CTL in these cultures. No obligate role for IFN-gamma was found. First, CTL activation in the presence of high dose cyclosporin A was partially reconstituted with IL-2, although no detectable IFN-gamma was produced in such cultures. In addition, IFN-gamma could not reconstitute cyclosporin-inhibited cultures. Second, inclusion of a high concentration of several distinct anti-IFN-gamma antibodies failed to inhibit MLR cultures. Third, CTL activation by stimulator cells inactivated by UV irradiation was reconstituted by IL-2, but not by IFN-gamma. These data do not support an autocrine role of IFN-gamma in CTL activation and confirm the importance of IL-2 in the primary murine MLR.
我们研究了两种淋巴因子，即白细胞介素-2（IL-2）和γ干扰素（IFN-γ）在小鼠原发性混合淋巴细胞反应（MLR）中的相对重要性。三条独立的证据表明，在这些培养物中，IL-2而非IFN-γ是CTL增殖和激活的相关淋巴因子。未发现IFN-γ有必然作用。首先，在高剂量环孢素A存在的情况下，用IL-2可部分重建CTL激活，尽管在此类培养物中未检测到IFN-γ产生。此外，IFN-γ无法重建环孢素抑制的培养物。其次，加入高浓度的几种不同抗IFN-γ抗体未能抑制MLR培养物。第三，紫外线照射灭活的刺激细胞激活的CTL可由IL-2重建，但不能由IFN-γ重建。这些数据不支持IFN-γ在CTL激活中的自分泌作用，并证实了IL-2在小鼠原发性MLR中的重要性。
