Peyronnard J M, Charron L F, Messier J P, Lavoie J, Faraco-Cantin F, Dubreuil M
Centre de recherche en sciences neurologiques, Faculté de médecine, Université de Montréal, Canada.
Muscle Nerve. 1988 Feb;11(2):108-13. doi: 10.1002/mus.880110204.
A method is described for histological localization of carbonic anhydrase (CA) in sections of frozen human muscle using the rapid and inexpensive histochemical technique of Hansson. Results obtained in normal subjects indicate clearly that CA reactive fibers are of type 1. Similarly, abnormalities seen with CA in the muscle biopsy of a patient presenting with type 1 fiber hypotrophy and preponderance duplicated almost exactly those observed with the actinomyosine adenosine triphosphatase and the reduced nicotinamide adenine dinucleotide dehydrogenase reactions. Observations of grouped CA-positive muscle fibers in a case of chronic neurogenic atrophy suggest that, like other enzymes, CA expression in muscle is under neurogenic control.
本文描述了一种使用汉森快速且经济的组织化学技术，在冷冻的人体肌肉切片中对碳酸酐酶（CA）进行组织学定位的方法。在正常受试者中获得的结果清楚地表明，CA反应性纤维属于1型。同样，在一名表现为1型纤维萎缩和优势的患者的肌肉活检中，CA检测到的异常几乎与用肌动球蛋白腺苷三磷酸酶和还原型烟酰胺腺嘌呤二核苷酸脱氢酶反应观察到的异常完全一致。在一例慢性神经源性萎缩病例中观察到成组的CA阳性肌纤维，这表明与其他酶一样，肌肉中的CA表达受神经源性控制。
