Department of Pathology, Kurume University, School of Medicine, Kurume, Japan.
Department of Hematology, Hokkaido University Faculty of Medicine, Sapporo, Japan.
PLoS One. 2018 Apr 11;13(4):e0194525. doi: 10.1371/journal.pone.0194525. eCollection 2018.
Polyploid chromosomes are those with more than two sets of homologous chromosomes. Polyploid chromosomal abnormalities are observed in various malignant tumors. The prognosis in such cases is generally poor. However, there are no studies examining the prognosis of diffuse large B-cell lymphoma (DLBCL) with polyploid chromosomal abnormalities. Therefore, we statistically compared the clinicopathological features between polyploid DLBCL and DLBCL without polyploid abnormalities. Herein, 51 polyploid DLBCL and 53 control (without polyploid chromosomal abnormalities) cases were examined. G-banding method was employed to define polyploidy by cytogenetic analysis. Subsequently, flow cytometric immunophenotyping and immunohistochemical staining were performed. Polyploid DLBCL was defined as DLBCL with either near-tetraploid or greater number of chromosomes, as detected by the G-band. In a survival analysis, a significantly worse overall survival (OS) was observed for polyploid DLBCL (p = 0.04; p = 0.02 in cases who received R-CHOP regimens). In a multivariate analysis of OS, polyploid chromosomal abnormalities were an independent prognostic factor. Our results suggest that polyploid chromosomal abnormalities detected through G-band may represent a new poor prognostic factor for DLBCL.
多倍体染色体是指具有两套以上同源染色体的染色体。多倍体染色体异常在各种恶性肿瘤中都有观察到。在这种情况下，预后通常较差。然而，目前还没有研究检查多倍体染色体异常的弥漫性大 B 细胞淋巴瘤（DLBCL）的预后。因此，我们对多倍体 DLBCL 和没有多倍体异常的 DLBCL 之间的临床病理特征进行了统计学比较。在此，检查了 51 例多倍体 DLBCL 和 53 例对照（无多倍体异常）病例。通过细胞遗传学分析，用 G 带法定义多倍体。随后进行流式细胞术免疫表型和免疫组织化学染色。通过 G 带检测，多倍体 DLBCL 被定义为具有近四倍体或更多染色体的 DLBCL。在生存分析中，多倍体 DLBCL 的总生存率（OS）明显较差（p = 0.04；接受 R-CHOP 方案的病例中 p = 0.02）。在 OS 的多变量分析中，多倍体染色体异常是一个独立的预后因素。我们的结果表明，通过 G 带检测到的多倍体染色体异常可能代表 DLBCL 的一个新的不良预后因素。
