Bueno Rafael D, Borges Leandro L, God Pedro I V Good, Piovesan Newton D, Teixeira Arlindo I, Cruz Cosme Damião, Barros Everaldo G DE
Universidade Federal de Viçosa/UFV, Instituto de Biotecnologia Aplicada à Agropecuária/Bioagro, Campus Viçosa, Avenida Ph Rolfs, s/n, 36570-900 Viçosa, MG, Brazil.
Universidade Federal de Viçosa/UFV, Instituto de Ciências Agrárias, Campus Rio Paranaíba, Rodovia AMG 230, Km 7, 38810-000 Rio Paranaíba, MG, Brazil.
An Acad Bras Cienc. 2018 Jan-Mar;90(1):205-217. doi: 10.1590/0001-3765201820140465.
Soybeans contain about 30% carbohydrate, mainly consisting of non-starch polysaccharides (NSP) and oligosaccharides. NSP are not hydrolyzed in the gastrointestinal tract of monogastric animals. These NSP negatively affect the development of these animals, especially the soluble fraction. This work aimed to establish a method to quantify NSP in soybeans, using high performance liquid chromatography (HPLC), and to estimate correlations between NSP, oligosaccharides, protein and oil. Sucrose, raffinose + stachyose, soluble and insoluble NSP contents were determined by HPLC. Oil and protein contents were determined by near-infrared spectroscopy (NIRS). The soluble PNAs content showed no significant correlation with protein, oil, sucrose and raffinose + stachyose contents, but oligosaccharides showed a negative correlation with protein content. These findings open up the possibility of developing cultivars with low soluble NSP content, aiming to develop feed for monogastric animals.
大豆含有约30%的碳水化合物，主要由非淀粉多糖（NSP）和寡糖组成。NSP在单胃动物的胃肠道中不被水解。这些NSP对这些动物的发育有负面影响，尤其是可溶性部分。这项工作旨在建立一种使用高效液相色谱（HPLC）定量大豆中NSP的方法，并估计NSP、寡糖、蛋白质和油脂之间的相关性。通过HPLC测定蔗糖、棉子糖+水苏糖、可溶性和不溶性NSP的含量。通过近红外光谱（NIRS）测定油脂和蛋白质含量。可溶性PNAs含量与蛋白质、油脂、蔗糖和棉子糖+水苏糖含量无显著相关性，但寡糖与蛋白质含量呈负相关。这些发现为培育低可溶性NSP含量的品种提供了可能性，旨在开发单胃动物饲料。
