Tu Yunjie, Shu Jingting, Ji Gaige, Zhang Ming, Zou Jianmin
Poultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou, Jiangsu, 225125, China.
Key lab of Poultry Genetics and Breeding in Jiangsu Province, Yangzhou, Jiangsu, 225125, China.
Asian-Australas J Anim Sci. 2018 Oct;31(10):1558-1564. doi: 10.5713/ajas.17.0627. Epub 2018 Apr 11.
We report monitoring conservation effect for a Chinese indigenous chicken (Langshan) breed using major histocompatibility complex (MHC) and DNA barcords.
The full length of MHC B-G gene and mitochondrial cytochrome oxidase I (COI) gene in generations 0, 5, 10, 15, 16, and 17 was measured using re-sequencing and sequencing procedures, respectively.
There were 292 single nucleotide polymorphisms of MHC B-G gene identified in six generations. Heterozygosity (He) and polymorphic information content (PIC) of MHC B-G gene in generations 10, 15, 16, and 17 remained stable. He and PIC of MHC B-G gene were different in six generations, with G10, G15, G16, G17 >G5>G0 (p<0.05). For the COI gene, there were five haplotypes in generations 0, 5, 10, 15, 16, and 17. Where Hap2 and Hap4 were the shared haplotypes, 164 individuals shared Hap2 haplotypes, while Hap1 and Hap3 were the shared haplotypes in generations 0 and 5 and Hap5 was a shared haplotype in generations 10, 15, 16, and 17. The sequence of COI gene in 6 generations was tested by Tajima's and D value, and the results were not significant, which were consistent with neutral mutation. There were no differences in generations 10, 15, 16, and 17for measured phenotypic traits. In other generations, for annual egg production, with G5, G10, G15, G16, G17>G0 (p<0.05). For age at the first egg and age at sexual maturity, with G10, G15, G16, G17>G5>G0 (p<0.05).
Combined with the results of COI gene DNA barcodes, MHC B-G gene, and phenotypic traits we can see that genetic diversity remained stable from generations 10 to 17 and the equimultiple random matching pedigrees conservation population conservation effect of Langshan chicken was effective as measured by these criteria.
我们报告了使用主要组织相容性复合体（MHC）和DNA条形码对中国本土鸡品种（狼山鸡）进行监测的保护效果。
分别使用重测序和测序程序测定第0、5、10、15、16和17代中MHC B-G基因的全长和线粒体细胞色素氧化酶I（COI）基因。
在六代中鉴定出MHC B-G基因的292个单核苷酸多态性。第10、15、16和17代中MHC B-G基因的杂合度（He）和多态信息含量（PIC）保持稳定。MHC B-G基因的He和PIC在六代中有所不同，G10、G15、G16、G17>G5>G0（p<0.05）。对于COI基因，第0、5、10、15、16和17代中有五种单倍型。其中Hap2和Hap4是共享单倍型，164个个体共享Hap2单倍型，而Hap1和Hap3是第0代和第5代的共享单倍型，Hap5是第10、15、16和17代的共享单倍型。通过Tajima's和D值对6代中COI基因的序列进行检验，结果不显著，这与中性突变一致。第10、15,、16和17代所测表型性状无差异。在其他代中，对于年产蛋量，G5、G10,、G15、G16、G17>G0（p<0.05）。对于开产日龄和性成熟日龄，G10、G15、G16、G17>G5>G0（p<0.05）。
结合COI基因DNA条形码、MHC B-G基因和表型性状的结果可以看出，从第10代到第17代遗传多样性保持稳定，按照这些标准衡量，狼山鸡的等量随机匹配家系保种群保护效果是有效的。
