Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.
Shenzhen College of Advanced Technology, University of Chinese Academy of Sciences, Shenzhen, China.
Reprod Sci. 2019 Oct;26(10):1360-1372. doi: 10.1177/1933719118765966. Epub 2018 Apr 11.
Obesity is a chronic multifactorial disease prevalent in many areas of the world and is a major cause of morbidity and mortality. In women, obesity increases the risks of both metabolic and reproductive diseases, such as diabetes and infertility. The mechanisms underlying these effects, especially in young women, are largely unknown. To explore these mechanisms, we established a high-fat diet (HFD) model of obesity in immature female mice. Microarray analysis of gene expression in ovaries and white adipose tissue identified a large number of differentially expressed genes (>1.3-fold change) in both tissues. In ovaries of the HFD group, there were 208 differentially expressed messenger RNAs (mRNAs), including 98 upregulated and 110 downregulated, and 295 differentially expressed lncRNAs (long non coding RNAs), including 63 upregulated and 232 downregulated. In white adipose tissue, there were 625 differentially expressed mRNAs, including 220 upregulated and 605 downregulated in the HFD group, and 1595 differentially expressed lncRNAs, including 1320 and 275 downregulated in the HFD group. Our results reveal significant differences between the transcriptomes of the HFD and control groups in both ovaries and white adipose tissue that provide clues to the molecular mechanisms of diet-induced female reproductive dysfunction and metabolic disorders, as well as biomarkers of risk for these disorders.
肥胖是一种在世界许多地区流行的慢性多因素疾病，也是发病率和死亡率的主要原因。在女性中，肥胖会增加代谢和生殖疾病的风险，如糖尿病和不孕。这些影响的机制，特别是在年轻女性中，很大程度上是未知的。为了探索这些机制，我们在未成熟的雌性小鼠中建立了高脂肪饮食（HFD）肥胖模型。对卵巢和白色脂肪组织中基因表达的微阵列分析鉴定出这两种组织中大量差异表达的基因（> 1.3 倍变化）。在 HFD 组的卵巢中，有 208 个差异表达的信使 RNA（mRNA），包括 98 个上调和 110 个下调，和 295 个差异表达的长非编码 RNA（lncRNA），包括 63 个上调和 232 个下调。在白色脂肪组织中，有 625 个差异表达的 mRNA，包括 HFD 组中 220 个上调和 605 个下调，以及 1595 个差异表达的 lncRNA，包括 HFD 组中 1320 个和 275 个下调。我们的研究结果揭示了 HFD 和对照组在卵巢和白色脂肪组织中的转录组之间存在显著差异，这些差异为饮食引起的女性生殖功能障碍和代谢紊乱的分子机制以及这些疾病风险的生物标志物提供了线索。
