Marine Ecology, GEOMAR Helmholtz Centre for Ocean Research Kiel, Germany
Marine Ecology, GEOMAR Helmholtz Centre for Ocean Research Kiel, Germany.
J R Soc Interface. 2018 Apr;15(141). doi: 10.1098/rsif.2017.0723.
confocal Raman microscopy (CRM), polarized light microscopy and Fourier transform infrared spectroscopy (FTIR) were used to determine if a significant amount of amorphous calcium carbonate (ACC) exists within larval shells of Baltic mytilid mussels (-like) and whether the amount of ACC varies during larval development. No evidence for ACC was found from the onset of shell deposition at 21 h post-fertilization (hpf) until 48 hpf. Larval shells were crystalline from 21 hpf onwards and exhibited CRM and FTIR peaks characteristic of aragonite. Prior to shell deposition at 21 hpf, no evidence for carbonates was observed through CRM. We further analysed the composition of larval shells in three other bivalve species, , and and observed no evidence for ACC, which is in contrast to previous work on the same species. Our findings indicate that larval bivalve shells are composed of crystalline aragonite and we demonstrate that conflicting results are related to sub-optimal measurements and misinterpretation of CRM spectra. Our results demonstrate that the common perception that ACC generally occurs as a stable and abundant precursor during larval bivalve calcification needs to be critically reviewed.
共聚焦拉曼显微镜（CRM）、偏光显微镜和傅里叶变换红外光谱（FTIR）被用于确定波罗的海贻贝幼虫贝壳（类）中是否存在大量非晶碳酸钙（ACC），以及 ACC 的含量是否在幼虫发育过程中发生变化。在受精后 21 小时（hpf）开始壳沉积到 48 hpf 期间，没有发现 ACC 的证据。幼虫贝壳从 21 hpf 开始结晶，并表现出方解石特征的 CRM 和 FTIR 峰。在 21 hpf 进行壳沉积之前，CRM 未观察到碳酸盐的证据。我们进一步分析了另外三种双壳类物种（、和）的幼虫贝壳的组成，没有发现 ACC 的证据，这与之前对同一物种的研究结果相反。我们的研究结果表明，幼虫双壳类贝壳由结晶文石组成，我们证明了相互矛盾的结果与次优的测量和 CRM 光谱的错误解释有关。我们的研究结果表明，普遍认为 ACC 在幼虫双壳类钙化过程中通常作为一种稳定且丰富的前体存在的观点需要进行批判性审查。
