Itoh H, Nakao K, Mukoyama M, Sugawara A, Saito Y, Morii N, Yamada T, Shiono S, Arai H, Imura H
Department of Medicine, Kyoto University School of Medicine, Japan.
Hypertension. 1988 Feb;11(2 Pt 2):I52-6. doi: 10.1161/01.hyp.11.2_pt_2.i52.
To elucidate the posttranslational processing of gamma-human atrial natriuretic polypeptide (human atrial natriuretic factor-[1-126]), which is a prohormone of alpha-human atrial natriuretic polypeptide (human atrial natriuretic factor-[99-126]), and the secretion of gamma-human atrial natriuretic polypeptide-derived peptides from the heart, we established a radioimmunoassay specific for the N-terminal sequence of gamma-human atrial natriuretic polypeptide, gamma-human atrial natriuretic polypeptide-(1-25), as well as a radioimmunoassay for alpha-human atrial natriuretic polypeptide. With the aid of the radioimmunoassays for gamma-human atrial natriuretic polypeptide-(1-25) and for alpha-human atrial natriuretic polypeptide, we detected 290 +/- 35.6 pg/ml of gamma-human atrial natriuretic polypeptide-(1-25)-like immunoreactivity in plasma from healthy humans, while the simultaneously determined plasma alpha-human atrial natriuretic polypeptide-like immunoreactivity level was 20.9 +/- 2.8 pg/ml. Correlation between the two values was significant. High performance gel permeation chromatographic analysis revealed that the plasma gamma-human atrial natriuretic polypeptide-(1-25)-like immunoreactivity was composed of a component (molecular weight, 10,000) without alpha-human atrial natriuretic polypeptide-like immunoreactivity, while the plasma alpha-human atrial natriuretic polypeptide-like immunoreactivity was composed of alpha-human atrial natriuretic polypeptide with a molecular weight of 3000. In patients with heart diseases, the plasma gamma-human atrial natriuretic polypeptide-(1-25)-like immunoreactivity level showed a concomitant and graded increase, with the plasma alpha-human atrial natriuretic polypeptide-like immunoreactivity level in agreement with the severity of the disease. There were significant positive correlations between the two immunoreactivity levels and right or left atrial pressure.(ABSTRACT TRUNCATED AT 250 WORDS)
为阐明γ-人心房利钠多肽（人心房利钠因子-[1-126]）的翻译后加工过程，其是α-人心房利钠多肽（人心房利钠因子-[99-126]）的前体激素，以及从心脏分泌的γ-人心房利钠多肽衍生肽，我们建立了一种对γ-人心房利钠多肽的N端序列γ-人心房利钠多肽-(1-25)特异的放射免疫测定法，以及一种对α-人心房利钠多肽的放射免疫测定法。借助γ-人心房利钠多肽-(1-25)和α-人心房利钠多肽的放射免疫测定法，我们在健康人血浆中检测到290±35.6 pg/ml的γ-人心房利钠多肽-(1-25)样免疫反应性，而同时测定的血浆α-人心房利钠多肽样免疫反应性水平为20.9±2.8 pg/ml。这两个值之间的相关性显著。高效凝胶渗透色谱分析显示，血浆γ-人心房利钠多肽-(1-25)样免疫反应性由一种无α-人心房利钠多肽样免疫反应性的成分（分子量10,000）组成，而血浆α-人心房利钠多肽样免疫反应性由分子量为3000的α-人心房利钠多肽组成。在心脏病患者中，血浆γ-人心房利钠多肽-(1-25)样免疫反应性水平呈现伴随性和分级性升高，血浆α-人心房利钠多肽样免疫反应性水平与疾病严重程度一致。这两种免疫反应性水平与右心房或左心房压力之间存在显著正相关。（摘要截短于250字）
