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常规使用的免疫测定法无法检测针对天然 NC1 六聚体和 IV 型胶原 α3 链 EA 表位的循环抗 GBM 抗体。

Routinely used immunoassays do not detect circulating anti-GBM antibodies against native NC1 hexamer and EA epitope of the α3 chain of type IV collagen.

机构信息

Laboratoire d'Immunologie, Pôle de Biologie, Centre Hospitalier Universitaire Grenoble Alpes, Grenoble, Cedex 9, France.

BNI team, TIMC-IMAG UMR5525 Université Grenoble Alpes - CNRS, Grenoble, Cedex 9, France.

出版信息

Eur J Immunol. 2018 Jun;48(6):1082-1084. doi: 10.1002/eji.201747324. Epub 2018 May 2.

DOI:10.1002/eji.201747324
PMID:29644627
Abstract

Detection of circulating anti-GBM antibodies has a key role for the diagnosis of Goodpasture syndrome but immunoassays using purified or recombinant alpha3(IV)NC1 as antigen do not recognize all anti-GBM antibodies. We show that anti-GBM antibodies directed against epitopes in their native conformation or cryptic epitopes are detected by indirect immunofluorescence.

摘要

检测循环中的抗肾小球基底膜抗体对 Goodpasture 综合征的诊断具有关键作用，但使用纯化或重组 alpha3(IV)NC1 作为抗原的免疫测定方法并不能识别所有的抗肾小球基底膜抗体。我们表明，针对其天然构象中的表位或隐匿表位的抗肾小球基底膜抗体可通过间接免疫荧光法检测。

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1
Routinely used immunoassays do not detect circulating anti-GBM antibodies against native NC1 hexamer and EA epitope of the α3 chain of type IV collagen.常规使用的免疫测定法无法检测针对天然 NC1 六聚体和 IV 型胶原 α3 链 EA 表位的循环抗 GBM 抗体。
Eur J Immunol. 2018 Jun;48(6):1082-1084. doi: 10.1002/eji.201747324. Epub 2018 May 2.
2
Circulating anti-glomerular basement membrane autoantibodies against α3(IV)NC1 undetectable by commercially available enzyme-linked immunosorbent assays.循环抗肾小球基底膜自身抗体针对α3(IV)NC1，无法通过市售酶联免疫吸附测定法检测。
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Natural anti-GBM antibodies from normal human sera recognize alpha3(IV)NC1 restrictively and recognize the same epitopes as anti-GBM antibodies from patients with anti-GBM disease.来自正常人血清的天然抗肾小球基底膜（GBM）抗体特异性识别α3（IV）NC1，并与抗GBM病患者的抗GBM抗体识别相同的表位。
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