Gottesman S R, Edington J M, Thorbecke G J
Department of Pathology, New York University School of Medicine, New York 10016.
J Immunol. 1988 Mar 15;140(6):1783-90.
The changes with age in three splenic suppressor cell populations were studied in C57BL/6 mice. Allospecific Ts cells and nonspecific non-T suppressor cells were both generated in vitro in allogeneic MLC. The presence of "pre-existing" suppressor cells in fresh spleen cells from normal mice was examined. Suppressor cell activities were assayed for their ability to suppress proliferation in a fresh allogeneic MLC after treatment to prevent their own proliferation. The ability to generate both specific and nonspecific suppressor cells decreased with age, whereas pre-existing suppressor cells were detected in spleens from the majority of the aged animals but not in spleens from young animals. The decrease in suppressor cell activity was not due to any requirement for age matching between donors of suppressor and target cells. The specific and nonspecific MLC-generated suppressor cells inhibited both the proliferative response in the assay MLC and the generation of cytotoxic cells. The pre-existing suppressor cells only suppressed the proliferative response and not the generation of cytotoxic cells. The changes seen with age in these suppressor cell populations suggest that the ability to generate suppression (both allospecific and nonspecific) to newly encountered Ag declines with age, whereas a resident splenic suppressor cell population accumulates over the lifetime of the animals.
在C57BL/6小鼠中研究了三种脾抑制细胞群体随年龄的变化。同种异体特异性Ts细胞和非特异性非T抑制细胞均可在同种异体混合淋巴细胞培养（MLC）中体外产生。检测了正常小鼠新鲜脾细胞中“预先存在”的抑制细胞。在处理以防止其自身增殖后，测定抑制细胞活性以评估其抑制新鲜同种异体MLC中增殖的能力。产生特异性和非特异性抑制细胞的能力随年龄下降，而在大多数老年动物的脾脏中检测到预先存在的抑制细胞，而在幼年动物的脾脏中未检测到。抑制细胞活性的下降并非由于抑制细胞供体与靶细胞之间年龄匹配的任何要求。由MLC产生的特异性和非特异性抑制细胞均抑制检测MLC中的增殖反应和细胞毒性细胞的产生。预先存在的抑制细胞仅抑制增殖反应，而不抑制细胞毒性细胞的产生。这些抑制细胞群体随年龄出现的变化表明，对新遇到的抗原产生抑制（同种异体特异性和非特异性）的能力随年龄下降，而脾脏中常驻抑制细胞群体在动物一生中会积累。
