使用OKT3杂交瘤细胞克隆激活CD3+人T淋巴细胞。
Use of OKT3 hybridoma cells to clonally activate CD3+ human T lymphocytes.
作者信息
Kabelitz D, Janssen O, Brucker C
机构信息
Department of Medical Microbiology and Immunology, University of Ulm, F.R.G.
出版信息
J Immunol Methods. 1988 Mar 16;107(2):211-6. doi: 10.1016/0022-1759(88)90220-7.
A simple and reliable method was developed to induce clonal growth of resting human T cells. In this limiting dilution (LD) culture system, responder cells (unseparated mononuclear cells, E rosette-purified T cells, or cell sorter-separated CD4+ and CD8+ subsets) were activated by irradiated anti-CD3-secreting (OKT3) hybridoma cells in the presence of exogenous IL-2 (crude culture supernatant or recombinant IL-2). Under these conditions, one out of 2-3 CD4+ and CD8+ T cells developed into a proliferating cell clone. Addition of recombinant IL-1 slightly enhanced the growth frequency and increased the clone size of CD4+ cells but did not affect the growth pattern of CD8+ cells.
开发了一种简单可靠的方法来诱导静息人T细胞的克隆生长。在这种有限稀释（LD）培养系统中，应答细胞（未分离的单核细胞、E花环纯化的T细胞或细胞分选仪分离的CD4+和CD8+亚群）在存在外源性IL-2（粗培养上清液或重组IL-2）的情况下，被经辐照的分泌抗CD3的（OKT3）杂交瘤细胞激活。在这些条件下，2-3个CD4+和CD8+T细胞中有一个发育成增殖性细胞克隆。添加重组IL-1可略微提高生长频率并增加CD4+细胞的克隆大小，但不影响CD8+细胞的生长模式。
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