2D LC HEART CUTTING ON-LINE OF PHENOLIC COMPOUNDS FROM THREE SPECIES OF THE GENUS SALIX.

The 2D LC heart-cutting on-line system was elaborated and employed to the analysis of simple phenols and polyphenols occurring in willow barks. Using the test-set of 52 compounds, the conditions of chromatographic separation in each dimension were optimized. The worked-up system was based on RP-separation in both dimensions and the use of different elution profiles on the first- and second-dimensional columns: gradient and multistep gradient elution, respectively. In all analyses the UV detector was used. Under optimized separation conditions slightly modified in respect to chemical composition of the each analyzed MeOH extracts from three willow barks: Salix daphnoides, S. puiputea and S. sachalinensis 'Sekka' the differences in phenolie acid and flavonoid compositions were revealed.