Shelver Weilin L, Smith David J
a USDA-ARS , Red River Valley Agricultural Research Center, Biosciences Research Laboratory , USA.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2018 Aug;35(8):1519-1529. doi: 10.1080/19440049.2018.1463568. Epub 2018 Jun 6.
Zilpaterol is a β-adrenergic agonist feed additive approved in the United States to increase weight gain and improve feed efficiency of cattle. A zilpaterol immunochromatographic assay was developed as an economical and user-friendly rapid detection method for zilpaterol and validated using urine and tissue samples derived from animal studies. The assay sensitivity was 1.7-23.2 ng g or mL across a variety of feed and animal matrices and did not cross-react with clenbuterol or ractopamine. No sample pre-treatment of cattle and sheep urine was needed, but horse urine and feed required dilution; skeletal muscle required solvent extraction prior to testing. Of 32 incurred sheep urine samples tested, zilpaterol content was correctly identified in all but 2 samples. Horse urine containing >10 ng mL of incurred zilpaterol residue (n = 48) was correctly identified as zilpaterol positive. The assay correctly identified 0-day withdrawal sheep muscle samples as zilpaterol positive and the control and longer withdrawal day sheep muscle samples as negative. Zilpaterol was demonstrated to be stable in horse urine when stored at -20°C for 7 years.
齐帕特罗是一种在美国被批准用于增加牛体重和提高饲料效率的β-肾上腺素能激动剂饲料添加剂。开发了一种齐帕特罗免疫色谱检测法，作为一种经济且用户友好的齐帕特罗快速检测方法，并使用来自动物研究的尿液和组织样本进行了验证。该检测方法在各种饲料和动物基质中的灵敏度为1.7 - 23.2纳克/克或毫升，且与克伦特罗或莱克多巴胺无交叉反应。牛和羊的尿液无需进行样品预处理，但马尿和饲料需要稀释；骨骼肌在检测前需要进行溶剂萃取。在检测的32份受污染的绵羊尿液样本中，除2份样本外，其余所有样本中的齐帕特罗含量均被正确识别。含有>10纳克/毫升受污染齐帕特罗残留的马尿（n = 48）被正确识别为齐帕特罗阳性。该检测方法正确地将0天停药期的绵羊肌肉样本识别为齐帕特罗阳性，而对照和更长停药期的绵羊肌肉样本识别为阴性。当在-20°C下储存7年时，齐帕特罗在马尿中被证明是稳定的。
