Ustuner Derya, Colak Emine, Dincer Murat, Tekin Neslihan, Burukoglu Donmez Dilek, Akyuz Fahrettin, Colak Ertugrul, Kolaç Umut Kerem, Entok Emre, Ustuner Mehmet Cengiz
1 Department of Medical Laboratory, Vocational School of Health Services, Eskisehir Osmangazi University , Eskisehir, Turkey .
2 Department of Medical Biology, Eskisehir Osmangazi University , Eskisehir, Turkey .
J Med Food. 2018 Sep;21(9):899-904. doi: 10.1089/jmf.2017.0143. Epub 2018 Mar 20.
The aim of this study is to examine the therapeutic effects of Olea europaea L. leaf extract on carbon tetrachloride (CCl)-induced liver damage in rats. In the experiments, 3- to 4-month-old 28 male Sprague-Dawley rats were divided into four groups: control, O. europaea leaf extract, CCl, and curative. The CCl and curative groups received CCl (0.2 mL/kg) intraperitoneally for 10 days to form hepatic injury. O. europaea (80 mg/kg) leaf extract was given orally to the curative group dissolved in distilled water the following 14 days. Hepatic and antioxidant enzyme levels, p53, caspase 3, lipid peroxidation marker malondialdehyde (MDA), and also DNA fragmentation levels were determined to establish oxidative stress in hepatic cell damage and its consequences. After formation of liver damage, oral administration of the O. europaea significantly reduced CCl-induced elevations of serum alkaline phosphatase, aspartate aminotransferase and alanine aminotransferase levels (P < .001), MDA levels of both blood (P < .001) and liver tissues (P < .001), DNA fragmentation (P < .001), p53 (P < .001), and caspase 3 (P < .001) levels of liver tissues. Also this administration in curative group significantly increased CCl-induced reductions of superoxide dismutase (SOD) (P < .001) and catalase (CAT) (P < .001) activity of blood samples and decreased SOD (P < .001) and CAT (P < .05) activity observed in liver tissue curative groups compared with CCl curative group. In CCl group, liver tissue samples exhibited remarkable damage because of CCl and reduction of these damages were observed in the curative group. Our results showed that O. europaea leaf extract was effective in reducing hepatic damage caused by CCl by reducing lipid peroxidation, regulating antioxidant enzymes, and minimizing DNA damage.
本研究旨在探讨油橄榄叶提取物对四氯化碳（CCl）诱导的大鼠肝损伤的治疗作用。实验中，将28只3至4月龄的雄性Sprague-Dawley大鼠分为四组：对照组、油橄榄叶提取物组、CCl组和治疗组。CCl组和治疗组腹腔注射CCl（0.2 mL/kg），持续10天以形成肝损伤。在接下来的14天里，治疗组口服溶于蒸馏水的油橄榄叶提取物（80 mg/kg）。测定肝酶和抗氧化酶水平、p53、半胱天冬酶3、脂质过氧化标志物丙二醛（MDA）以及DNA片段化水平，以确定肝细胞损伤中的氧化应激及其后果。肝损伤形成后，口服油橄榄叶提取物可显著降低CCl诱导的血清碱性磷酸酶、天冬氨酸转氨酶和丙氨酸转氨酶水平升高（P < 0.001），血液（P < 0.001）和肝组织（P < 0.001）的MDA水平、DNA片段化（P < 0.001）、肝组织p53（P < 0.001）和半胱天冬酶3（P < 0.001）水平。此外，治疗组的这种给药方式还显著提高了CCl诱导降低的血样中超氧化物歧化酶（SOD）（P < 0.001）和过氧化氢酶（CAT）（P < 0.001）活性，并降低了与CCl治疗组相比在肝组织治疗组中观察到的SOD（P < 0.001）和CAT（P < 0.05）活性。在CCl组中，肝组织样本因CCl而表现出明显损伤，而在治疗组中观察到这些损伤有所减轻。我们的结果表明，油橄榄叶提取物通过减少脂质过氧化、调节抗氧化酶和最小化DNA损伤，有效减轻了CCl引起的肝损伤。
