Institut National de l'environnement Industriel et des Risques (INERIS), Unité d'Ecotoxicologie in vitro et in vivo, UMR-I SEBIO 02, BP 2, 60550 Verneuil-en-Halatte, France.
Université de Bordeaux, EPOC-UMR 5805, Laboratoire de Physico- et Toxico-Chimie de l'environnement (LPTC), 33405 Talence, France.
Int J Mol Sci. 2018 Apr 12;19(4):1175. doi: 10.3390/ijms19041175.
Triclosan (TCS), an antimicrobial agent widely found in the aquatic environment, is suspected to act as an endocrine disrupting compound, however mechanistic information is lacking in regards to aquatic species. This study assessed the ability of TCS to interfere with estrogen receptor (ER) transcriptional activity, in zebrafish-specific in vitro and in vivo reporter gene assays. We report that TCS exhibits a lack of either agonistic or antagonistic effects on a panel of ER-expressing zebrafish (ZELH-zfERα and -zfERβ) and human (MELN) cell lines. At the organism level, TCS at concentrations of up to 0.3 µM had no effect on ER-regulated brain aromatase gene expression in transgenic cyp19a1b-GFP zebrafish embryos. At a concentration of 1 µM, TCS interfered with the E2 response in an ambivalent manner by potentializing a low E2 response (0.625 nM), but decreasing a high E2 response (10 nM). Altogether, our study suggests that while modulation of ER-regulated genes by TCS may occur in zebrafish, it does so irrespective of a direct binding and activation of zfERs.
三氯生（TCS）是一种广泛存在于水环境中的抗菌剂，被怀疑是一种内分泌干扰化合物，但关于水生生物的机制信息还很缺乏。本研究评估了 TCS 干扰鱼类雌激素受体（ER）转录活性的能力，采用了斑马鱼特有的体外和体内报告基因检测方法。我们报告称，TCS 对一系列表达 ER 的斑马鱼（ZELH-zfERα 和 -zfERβ 以及人类 MELN）细胞系既没有激动作用也没有拮抗作用。在个体水平上，浓度高达 0.3 µM 的 TCS 对转基因 cyp19a1b-GFP 斑马鱼胚胎中 ER 调节的脑芳香酶基因表达没有影响。在 1 µM 的浓度下，TCS 以一种模棱两可的方式干扰了 E2 的反应，既能增强低浓度 E2（0.625 nM）的反应，又能降低高浓度 E2（10 nM）的反应。总的来说，我们的研究表明，尽管 TCS 可能会调节斑马鱼的 ER 调节基因，但它不是通过直接结合和激活 zfER 来实现的。
