Algal Processing Group, Department of Chemical Engineering, The University of Melbourne, Parkville, Victoria 3010, Australia.
Algal Processing Group, Department of Chemical Engineering, The University of Melbourne, Parkville, Victoria 3010, Australia.
Bioresour Technol. 2018 Jul;260:338-347. doi: 10.1016/j.biortech.2018.03.129. Epub 2018 Apr 1.
A single-step method for transesterifying and recovering lipids in concentrated slurries (ca 20% w/w solids) of ruptured microalgae is presented. A soluble Rhizomucor miehei lipase (RML) was used to directly transesterify the lipids in the marine microalgae Nannochloropsis salina. This allowed both triglycerides (TAG) and polar saponifiable lipids to be recovered as fatty acid methyl esters (FAME) using a nonpolar solvent (hexane). Up to 90 wt% of the total saponifiable lipids (SL) were converted to FAME within 24 h, approximately 75% of which was recovered in the hexane by centrifugation. Two pathways for the conversion and recovery of polar lipids were identified. The water in the slurry buffered against potential lipase inhibition by methanol, but necessitated a high methanol dose for maximal FAME conversion. Nonetheless the method enables the recovery of polar lipids as FAME while avoiding the need for both drying of the biomass and a downstream transesterification step.
本文介绍了一种在破裂微藻的高浓度悬浮液（约 20%w/w 固体）中进行酯交换和回收脂质的一步法。使用可溶的米根霉脂肪酶（RML）直接对海洋微藻盐藻中的脂质进行酯交换。这使得使用非极性溶剂（己烷）可以将甘油三酯（TAG）和极性可皂化脂质都回收为脂肪酸甲酯（FAME）。在 24 小时内，总可皂化脂质（SL）中多达 90wt%转化为 FAME，其中约 75%通过离心回收于己烷相中。确定了极性脂质转化和回收的两种途径。悬浮液中的水缓冲了甲醇对脂肪酶的潜在抑制作用，但需要高剂量的甲醇才能实现最大的 FAME 转化率。尽管如此，该方法能够将极性脂质回收为 FAME，同时避免了对生物质进行干燥和下游酯交换步骤的需要。
