Stockinger B, Hausmann B
Basel Institute for Immunology, Switzerland.
Eur J Immunol. 1988 Feb;18(2):249-53. doi: 10.1002/eji.1830180211.
The question has been addressed whether the endogenous B cell population of a mouse can be induced to secrete antibodies specific for a self antigen present in serum. The antigen studied was the fifth component of mouse complement (C5). Nude BALB/c mice which are C5 sufficient were used as a source of potentially C5-reactive B cells and endogenous serum C5 provided the antigenic stimulus. We purposely avoided immunization with C5 in adjuvant. T cells from C5-deficient mice which lack this component in serum and are therefore not tolerant of C5 were injected into nude mice as a source of T cell help for anti-C5 reactive B cells. Control groups received T cells from C5-sufficient euthymic donors, which are tolerant of C5. Initiation of a response to C5 was monitored by testing the hemolytic function of serum. Reduction of C5-dependent hemolysis was observed in sera of mice which had received T cells from C5-deficient donors. Recipients of T cells from C5-sufficient donors maintained normal hemolytic complement levels throughout the test period of 45 days. Reduction of functional complement levels correlated with the presence of immune complexes of anti-C5/C5. C5-specific antibodies were mainly IgG1 and carried the IgG1 allotype of BALB/c providing unequivocal evidence that they were derived from the endogenous B cell population of the C5-sufficient host.
小鼠体内的内源性B细胞群体能否被诱导分泌针对血清中自身抗原的特异性抗体，这一问题已得到研究。所研究的抗原是小鼠补体的第五成分（C5）。具有足够C5的裸BALB/c小鼠被用作潜在的C5反应性B细胞来源，内源性血清C5提供抗原刺激。我们特意避免用佐剂中的C5进行免疫。将血清中缺乏该成分且因此对C5不耐受的C5缺陷小鼠的T细胞注入裸鼠，作为抗C5反应性B细胞的T细胞辅助来源。对照组接受来自对C5耐受的具有正常胸腺的C5充足供体的T细胞。通过检测血清的溶血功能来监测对C5反应的启动。在接受来自C5缺陷供体的T细胞的小鼠血清中观察到C5依赖性溶血减少。在45天的测试期内，接受来自C5充足供体的T细胞的受体维持正常的溶血补体水平。功能性补体水平的降低与抗C5/C5免疫复合物的存在相关。C5特异性抗体主要为IgG1，并携带BALB/c的IgG1同种异型，这提供了明确的证据，表明它们源自C5充足宿主的内源性B细胞群体。
