Nanotechnology and Advanced Materials Central Lab., Agriculture Research Center, Giza, Egypt; Regional Center for Food and Feed, Agriculture Research Center, Giza, Egypt.
Nanotechnology and Advanced Materials Central Lab., Agriculture Research Center, Giza, Egypt.
Int J Biol Macromol. 2018 Aug;115:358-366. doi: 10.1016/j.ijbiomac.2018.04.055. Epub 2018 Apr 11.
Chitosan nanoparticles have many applications, such as gene and drug delivery, due to their biocompatibility. Chitosan nanoparticles are currently produced by dissolution in acetic acid that affects the biocompatibility at acidic pH. Here, we synthesized and characterized chitosan (CS) and ascorbate chitosan (AsCS) nanoparticles and investigated their cytotoxic effects, internalization, and distribution in the human colon carcinoma cell line using confocal laser scanning microscopy (CLSM). The CS and AsCS nanoparticles were spherical with average particle sizes of 44±8.4nm and 87±13.6nm, respectively. CS nanoparticles were taken up by the cells and showed dose-dependent cytotoxicity. By contrast, AsCS nanoparticles were not internalized and showed no cytotoxicity. Therefore, AsCS nanoparticles are more biocompatible than CS nanoparticles and may be more suitable for extracellular drug delivery.
壳聚糖纳米粒具有生物相容性，因此有许多应用，如基因和药物传递。壳聚糖纳米粒目前是通过溶解在醋酸中生产的，这会影响在酸性 pH 值下的生物相容性。在这里，我们合成并表征了壳聚糖（CS）和抗坏血酸壳聚糖（AsCS）纳米粒，并使用共聚焦激光扫描显微镜（CLSM）研究了它们在人结肠癌细胞系中的细胞内摄取、分布和细胞毒性作用。CS 和 AsCS 纳米粒呈球形，平均粒径分别为 44±8.4nm 和 87±13.6nm。CS 纳米粒被细胞摄取，并表现出剂量依赖性的细胞毒性。相比之下，AsCS 纳米粒未被内化，没有表现出细胞毒性。因此，AsCS 纳米粒比 CS 纳米粒更具生物相容性，可能更适合细胞外药物传递。
