State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection , Chinese Academy of Agricultural Sciences , No. 2 West Yuanmingyuan Road , Haidian District, Beijing 100193 , People's Republic of China.
Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center , Chinese Academy of Fishery Sciences , Wuxi , Jiangsu 214081 , People's Republic of China.
J Agric Food Chem. 2018 May 2;66(17):4336-4344. doi: 10.1021/acs.jafc.8b01070. Epub 2018 Apr 19.
As a result of the large-scale planting of transgenic Bacillus thuringiensis (Bt) crops, fish would be exposed to freely soluble Bt insecticidal protein(s) that are released from Bt crop tissues into adjacent bodies of water or by way of direct feeding on deposited plant material. To assess the safety of two Bt proteins Cry1C and Cry2A to fish, we used zebrafish as a representative species and exposed their embryos to 0.1, 1, and 10 mg/L of the two Cry proteins until 132 h post-fertilization and then several developmental, biochemical, and molecular parameters were evaluated. Chlorpyrifos (CPF), a known toxicant to aquatic organisms, was used as a positive control. Although CPF exposure resulted in significant developmental, biochemical, and molecular changes in the zebrafish embryos, there were almost no significant differences after Cry1C or Cry2A exposure. Thus, we conclude that zebrafish embryos are not sensitive to Cry1C and Cry2A insecticidal proteins at test concentrations.
由于大规模种植转基因苏云金芽孢杆菌（Bt）作物，鱼类将接触到从 Bt 作物组织释放到附近水体中的自由溶解的 Bt 杀虫蛋白，或者通过直接摄食沉积的植物材料。为了评估两种 Bt 蛋白 Cry1C 和 Cry2A 对鱼类的安全性，我们使用斑马鱼作为代表性物种，将其胚胎暴露于 0.1、1 和 10mg/L 的两种 Cry 蛋白中，直到受精后 132 小时，然后评估了几个发育、生化和分子参数。毒死蜱（CPF）是一种已知对水生生物有毒的物质，被用作阳性对照。尽管 CPF 暴露导致斑马鱼胚胎发生显著的发育、生化和分子变化，但在暴露于 Cry1C 或 Cry2A 后几乎没有显著差异。因此，我们得出结论，在测试浓度下，斑马鱼胚胎对 Cry1C 和 Cry2A 杀虫蛋白不敏感。
