State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Agro-Product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.
Engineering Research Centre of Ecology and Agricultural Use of Wetland, Ministry of Education, College of Animal Science, Yangtze University, Jingzhou, 434025, China.
Sci Rep. 2023 Apr 3;13(1):5445. doi: 10.1038/s41598-023-31072-2.
Transgenic soybean is one of the most planted crops for human food and animal feed. The channel catfish (Ictalurus punctatus) is an important aquatic organism cultured worldwide. In this study, the effect of six different soybean diets containing: two transgenic soybeans expressing different types of cp4-epsps, Vip3Aa and pat genes (DBN9004 and DBN8002), their non-transgenic parent JACK, and three conventional soybean varieties (Dongsheng3, Dongsheng7, and Dongsheng9) was investigated in juvenile channel catfish for eight weeks, and a safety assessment was performed. During the experiment, no difference in survival rate was observed in six groups. The hepatosomatic index (HSI) and condition factor (CF) showed no significant difference. Moreover, comparable feed conversion (FC), feeding rate (FR), and feed conversion ratio (FCR) were found between transgenic soybean and JACK groups. Assessment of growth performance showed that the weight gain rate (WGR) and specific growth rate (SGR) of channel catfish were consistent. In addition, there were no changes in enzyme activity indexes (lactate dehydrogenase (LDH), total antioxidant capacity (T-AOC), aspartate aminotransferase (AST) and alanine aminotransferase (ALT)) in channel catfish among treatments. The research provided an experimental basis for the aquaculture feed industry to employ transgenic soybean DBN9004 and DBN8002 for commercial purposes.
转基因大豆是人类食品和动物饲料的主要种植作物之一。斑点叉尾鮰（Ictalurus punctatus）是世界范围内广泛养殖的重要水生生物。本研究将转基因大豆 DBN9004 和 DBN8002 及其非转基因亲本 JACK，以及三种传统大豆品种（东升 3 号、东升 7 号和东升 9 号）制成的六种不同的大豆饲料喂养幼龄斑点叉尾鮰 8 周，对其进行安全性评估。实验期间，6 个组的存活率无差异。肝体比（HSI）和体况系数（CF）也没有显著差异。此外，转基因大豆组和 JACK 组的饲料转化率（FC）、摄食率（FR）和饲料转化率（FCR）相当。生长性能评估表明，斑点叉尾鮰的增重率（WGR）和特定生长率（SGR）一致。此外，处理组之间斑点叉尾鮰的酶活性指标（乳酸脱氢酶（LDH）、总抗氧化能力（T-AOC）、天冬氨酸氨基转移酶（AST）和丙氨酸氨基转移酶（ALT））没有变化。该研究为水产养殖饲料行业采用转基因大豆 DBN9004 和 DBN8002 进行商业生产提供了实验依据。
