Ramautar Rawi
Leiden Academic Center for Drug Research, Leiden University, Leiden, The Netherlands.
Methods Mol Biol. 2018;1738:183-192. doi: 10.1007/978-1-4939-7643-0_12.
Capillary electrophoresis (CE) is well suited for the profiling of highly polar and charged metabolites as compounds are separated on the basis of their charge-to-size ratio. The protocol presented here is based on using a recently developed sheathless interfacing design, i.e., a porous tip interface, for coupling CE to electrospray ionization mass spectrometry (MS). It is demonstrated that sheathless CE-MS employing a bare fused-silica capillary at low-pH separation conditions can be used for the profiling of both cationic and anionic metabolites by only switching the MS detection and electrophoretic separation voltage polarity. The proposed sheathless CE-MS protocol allows efficient and sensitive profiles to be obtained for a broad array of charged metabolites, including amino acids, organic acids, nucleotides, and sugar phosphates, in various biological samples, such as urine and extracts of the glioblastoma cell line.
毛细管电泳（CE）非常适合用于分析高极性和带电代谢物，因为化合物是根据其电荷与大小之比进行分离的。本文介绍的方案基于使用一种最近开发的无鞘接口设计，即多孔尖端接口，用于将CE与电喷雾电离质谱（MS）联用。结果表明，在低pH分离条件下使用裸露熔融石英毛细管的无鞘CE-MS，仅通过切换MS检测和电泳分离电压极性，就可用于分析阳离子和阴离子代谢物。所提出的无鞘CE-MS方案能够在各种生物样品（如尿液和成胶质细胞瘤细胞系提取物）中，高效且灵敏地分析包括氨基酸、有机酸、核苷酸和糖磷酸盐在内的多种带电代谢物。
