Rangel-Mendoza J, Hernández-García J, Álvarez-González C A, Guerrero-Zárate R, Zenteno-Ruiz C E, López-Luna M A
División Académica de Ciencias Biológicas, Universidad Juárez Autónoma de Tabasco, Villahermosa, Tabasco, México.
J Anim Physiol Anim Nutr (Berl). 2018 Aug;102(4):1102-1110. doi: 10.1111/jpn.12889. Epub 2018 Apr 14.
Functional characteristics of digestive proteases and in vitro digestibility of several protein sources were studied in hatchlings of Central American river turtles, Dermatemys mawii. Acidic and alkaline proteases from the stomach and intestines were used, and optimums for acidic proteases were registered at 55°C and pH 2, while alkaline proteases were found at 55°C and pH 9. Ten protein ingredients, both vegetable and animal sources, were explored by the pH-STAT method, only for alkaline proteases. The degree of hydrolysis was at its highest for squid meal and lowest for blood meal, while the total free amino acids level was at its highest for squid meal, and lowest for wheat gluten meal. Our results indicate that D. mawii has a broad capacity to digest both animal and vegetable sources, and suggests some ingredients more suitable to design artificial diets for this species.
研究了中美河龟（Dermatemys mawii）幼体消化蛋白酶的功能特性以及几种蛋白质来源的体外消化率。使用了来自胃和肠道的酸性和碱性蛋白酶，酸性蛋白酶的最佳温度和pH值分别为55°C和pH 2，而碱性蛋白酶则为55°C和pH 9。采用pH-STAT法仅对碱性蛋白酶研究了10种蛋白质成分，包括植物和动物来源。水解程度以鱿鱼粉最高，血粉最低，而总游离氨基酸水平以鱿鱼粉最高，小麦面筋粉最低。我们的结果表明，中美河龟具有消化动物和植物来源食物的广泛能力，并提出了一些更适合为该物种设计人工饲料的成分。
