Department of General Surgery, The Second Affiliated Hospital of Nanchang University, 330006 Nanchang, Jiangxi, China.
The First Clinical Medical College, Nanchang University, Nanchang, Jiangxi, China.
Exp Cell Res. 2018 Jul 1;368(1):24-36. doi: 10.1016/j.yexcr.2018.04.009. Epub 2018 Apr 11.
The human rhomboid family-1 gene (RHBDF1) is an oncogene in breast and head and neck squamous cancers. Here, we show that RHBDF1 plays a significant role in colorectal cancer (CRC) formation and that the RHBDF1 expression level is higher in CRC than in corresponding normal tissues. Moreover, RHBDF1 promotes cell proliferation, invasion and migration in vitro. Furthermore, through overexpression and silencing of RHBDF1 and the mediator complex, our study demonstrates that RHBDF1 may positively regulate adenomatous polyposis coli (APC) in the Wnt/β-catenin signalling pathway to increase the expression levels of MMP-14 and Twist, which act as important epithelial-to-mesenchymal transition (EMT) stimulating factors. Additionally, RHBDF1 may regulate c-myc and CyclinD1 expression to influence cell proliferation. Finally, RHBDF1 overexpression and silencing influence CRC growth in BALB/c nude mice. In summary, our findings demonstrate that the regulatory effects of RHBDF1 on EMT and on cell proliferation are partially attributable to the Wnt/β-catenin signalling pathway.
人类菱形家族-1 基因（RHBDF1）是乳腺癌和头颈部鳞状细胞癌中的癌基因。在这里，我们表明 RHBDF1 在结直肠癌（CRC）的形成中起着重要作用，并且 RHBDF1 在 CRC 中的表达水平高于相应的正常组织。此外，RHBDF1 在体外促进细胞增殖、侵袭和迁移。此外，通过过表达和沉默 RHBDF1 以及中介复合物，我们的研究表明 RHBDF1 可能通过 Wnt/β-catenin 信号通路正向调节腺瘤性结肠息肉病（APC），以增加 MMP-14 和 Twist 的表达水平，这两者作为重要的上皮-间充质转化（EMT）刺激因子。此外，RHBDF1 可能通过调节 c-myc 和 CyclinD1 的表达来影响细胞增殖。最后，RHBDF1 的过表达和沉默影响 BALB/c 裸鼠 CRC 的生长。总之，我们的研究结果表明，RHBDF1 对 EMT 和细胞增殖的调节作用部分归因于 Wnt/β-catenin 信号通路。
