花生皮原花青素诱导人前列腺癌细胞 DU145 增殖抑制。
Procyanidin from peanut skin induces antiproliferative effect in human prostate carcinoma cells DU145.
机构信息
College of Food Science and Technology, Huazhong Agricultural University, Wuhan, China.
College of Food Science and Technology, Huazhong Agricultural University, Wuhan, China; Wuhan Engineering Research Center of Bee Products on Quality and Safety Control, Wuhan, China; Key Laboratory of Environment Correlative Dietology (Huazhong Agricultural University), Ministry of Education, Wuhan, China.
出版信息
Chem Biol Interact. 2018 May 25;288:12-23. doi: 10.1016/j.cbi.2018.04.008. Epub 2018 Apr 11.
In this study, the antiproliferative activity of peanut skin procyanidins (PSP) and six fractions (PSP-1∼6) isolated from PSP by several chromatographic steps on the human prostate cancer DU145 cells were evaluated. The results showed that PSP and PSP-1∼6 significantly inhibited the proliferation of DU145 cells. PSP-2 was the most effective fraction, which was identified as procyanidin B mainly and procyanidin dimer [(E)C-luteolin or keampferol] secondarily. Moreover, the mechanism of antiproliferative activity of PSP-2 was investigated. It was observed that PSP-2 induced apoptotic cell death and cell cycle arrest at S phase in DU145 cells. PSP-2 caused the increase of intracellular ROS level and the decrease of Bcl-2/Bax ratio, and triggered the activation of p53 and caspases-3 in DU145 cells. Our findings demonstrated that procyanidins from peanut skin have the potential to be developed as an anti-prostate cancer agent.
本研究评估了花生皮原花青素（PSP）及其通过几种色谱步骤从 PSP 分离得到的六个馏分（PSP-1∼6）对人前列腺癌细胞 DU145 的抗增殖活性。结果表明，PSP 和 PSP-1∼6 显著抑制 DU145 细胞的增殖。PSP-2 是最有效的馏分，被鉴定为主要原花青素 B 和次要原花青素二聚体[(E)C-芦丁或槲皮素]。此外，还研究了 PSP-2 的抗增殖活性的机制。结果表明，PSP-2 在 DU145 细胞中诱导凋亡细胞死亡和细胞周期停滞在 S 期。PSP-2 导致细胞内 ROS 水平升高和 Bcl-2/Bax 比例降低，并触发 DU145 细胞中 p53 和 caspase-3 的激活。我们的研究结果表明，花生皮中原花青素具有开发为抗前列腺癌药物的潜力。
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