College of Food Science and Technology, Huazhong Agricultural University, Wuhan, China.
College of Food Science and Technology, Huazhong Agricultural University, Wuhan, China; Wuhan Engineering Research Center of Bee Products on Quality and Safety Control, Wuhan, China; Key Laboratory of Environment Correlative Dietology (Huazhong Agricultural University), Ministry of Education, Wuhan, China.
Chem Biol Interact. 2018 May 25;288:12-23. doi: 10.1016/j.cbi.2018.04.008. Epub 2018 Apr 11.
In this study, the antiproliferative activity of peanut skin procyanidins (PSP) and six fractions (PSP-1∼6) isolated from PSP by several chromatographic steps on the human prostate cancer DU145 cells were evaluated. The results showed that PSP and PSP-1∼6 significantly inhibited the proliferation of DU145 cells. PSP-2 was the most effective fraction, which was identified as procyanidin B mainly and procyanidin dimer [(E)C-luteolin or keampferol] secondarily. Moreover, the mechanism of antiproliferative activity of PSP-2 was investigated. It was observed that PSP-2 induced apoptotic cell death and cell cycle arrest at S phase in DU145 cells. PSP-2 caused the increase of intracellular ROS level and the decrease of Bcl-2/Bax ratio, and triggered the activation of p53 and caspases-3 in DU145 cells. Our findings demonstrated that procyanidins from peanut skin have the potential to be developed as an anti-prostate cancer agent.
本研究评估了花生皮原花青素（PSP）及其通过几种色谱步骤从 PSP 分离得到的六个馏分（PSP-1∼6）对人前列腺癌细胞 DU145 的抗增殖活性。结果表明，PSP 和 PSP-1∼6 显著抑制 DU145 细胞的增殖。PSP-2 是最有效的馏分，被鉴定为主要原花青素 B 和次要原花青素二聚体[(E)C-芦丁或槲皮素]。此外，还研究了 PSP-2 的抗增殖活性的机制。结果表明，PSP-2 在 DU145 细胞中诱导凋亡细胞死亡和细胞周期停滞在 S 期。PSP-2 导致细胞内 ROS 水平升高和 Bcl-2/Bax 比例降低，并触发 DU145 细胞中 p53 和 caspase-3 的激活。我们的研究结果表明，花生皮中原花青素具有开发为抗前列腺癌药物的潜力。
