异基因造血干细胞移植后通过短串联重复序列分析评估脑脊液中的供体细胞嵌合率。
Donor cell chimerism evaluation in cerebrospinal fluid by short tandem repeat analysis following allogeneic hematopoietic stem cell transplantation.
作者信息
Spruit Jessica L, Fagoaga Omar R, Savaşan Süreyya
机构信息
Pediatric Blood and Marrow Transplantation, Children's Hospital of Michigan, 3901 Beaubien, Detroit, MI 48201, USA.
Histocompatibility and Immunogenetics Lab, DMC University Laboratories, Hutzel/KEI Building, 4707 Saint Antoine Street, Detroit, MI 48201, USA; Wayne State University School of Medicine, 540 E Canfield Ave, Detroit, MI 48201, USA.
出版信息
Hum Immunol. 2018 Jul;79(7):537-538. doi: 10.1016/j.humimm.2018.04.001. Epub 2018 Apr 11.
Cerebrospinal fluid (CSF) cell count, morphology and flow cytometric evaluations are used to investigate central nervous system (CNS) involvement in leukemia/lymphoma. We performed CSF short tandem repeat (STR) analysis to monitor CSF chimerism status and evaluate for disease involvement in 11 asymptomatic pediatric allogeneic hematopoietic stem cell transplantation (HSCT) recipients with hemato-lymphoid neoplasms and high risk for or history of CNS involvement. Eighteen (64%) of the samples with median CSF cell count of 1/mm with 90% lymphocytes gave conclusive STR results, suggesting that this DNA-based method can be used in monitoring CSF chimerism status after HSCT with an acceptable yield.
脑脊液（CSF）细胞计数、形态学及流式细胞术评估用于研究白血病/淋巴瘤患者的中枢神经系统（CNS）受累情况。我们对11例患有血液淋巴系统肿瘤且有CNS受累高风险或CNS受累病史的无症状儿科异基因造血干细胞移植（HSCT）受者进行了脑脊液短串联重复序列（STR）分析，以监测脑脊液嵌合状态并评估疾病受累情况。18份样本（64%）的脑脊液细胞计数中位数为1/mm，淋巴细胞占90%，得到了明确的STR结果，表明这种基于DNA的方法可用于监测HSCT后脑脊液嵌合状态，且产出率可接受。
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