The School of Pharmacy, University of Reading, Reading RG6 6AD, UK.
Faculty of Science & Technology, University of Westminster, 115 New Cavendish Street, London W1W 6UW, UK.
Eur J Pharm Biopharm. 2018 Jul;128:27-35. doi: 10.1016/j.ejpb.2018.04.006. Epub 2018 Apr 11.
Ciprofloxacin (CFX) is a fluoroquinolone antibiotic used as a first line treatment against infections caused by Pseudomonas aeruginosa and Streptococcus pneumonia that are commonly acquired by cystic fibrosis (CF) patients. However, no inhalation formulation is currently available for ciprofloxacin. Hybrid silica coated silver nanoparticles were prepared using Stöber reaction and the optimum ratio of chitosan and sodium tripolyphosphate was used to encapsulate CFX. Particle deposition was assessed in vitro using twin stage impinger while antimicrobial activity was evaluated based on the planktonic growth of P. aeruginosa as well as against P. aeruginosa sp biofilm formation. In vitro deposition results showed significant deposition in stage 2 using twin stage impinger (TSI) (∼70%). Compared to CFX, the formed hybrid nanoparticles were 3-4 folds more effective against inhibiting growth and biofilm formation by P. aeruginosa PAO1 and P. aeruginosa NCTC 10662.
环丙沙星（CFX）是一种氟喹诺酮类抗生素，用作囊性纤维化（CF）患者常感染的铜绿假单胞菌和肺炎链球菌引起的感染的一线治疗药物。然而，目前没有环丙沙星的吸入制剂。使用Stöber 反应制备了混合二氧化硅包覆的银纳米粒子，并使用壳聚糖和三聚磷酸钠的最佳比例来包封 CFX。使用双级冲击器评估体外颗粒沉积，同时根据铜绿假单胞菌的浮游生长以及对铜绿假单胞菌 sp 生物膜形成的抗微生物活性来评估抗菌活性。体外沉积结果表明，在双级冲击器（TSI）中，第二级有明显的沉积（约 70%）。与 CFX 相比，形成的混合纳米粒子对抑制铜绿假单胞菌 PAO1 和铜绿假单胞菌 NCTC 10662 的生长和生物膜形成的效果要强 3-4 倍。
