Department of Endocrinology, The First Affiliated Hospital of Bengbu Medical College, Bengbu 233004, China.
Department of Pharmacology, School of Basic Medical Science, Nanjing Medical University, Nanjing 210009, China.
Int Immunopharmacol. 2018 Jun;59:141-147. doi: 10.1016/j.intimp.2018.03.035. Epub 2018 Apr 11.
The current study was designed to investigate the protective role of geniposide (GE) in liver injury in diabetic C57BL/KsJ-db/db mice and to explore the underlying mechanisms. The oral glucose tolerance test was performed, and the levels of insulin, alanine aminotransferase (ALT), aspartate aminotransaminase (AST), total cholesterol (TC) and triglyceride (TG) were determined. The levels of the pro-inflammatory cytokines interleukin (IL)-1β, IL-6 and tumour necrosis factor-α were decreased by GE, metformin and fasudil in diabetic db/db mice. Western blotting analysis showed that the expression levels of Rho, ROCK1, ROCK2, p-NF-κBp65 and p-IκBα were significantly reversed by GE treatment. These findings demonstrated that GE exhibits a protective effect on diabetic hepatic inflammation.
本研究旨在探讨京尼平苷（GE）在糖尿病 C57BL/KsJ-db/db 小鼠肝损伤中的保护作用，并探讨其潜在机制。进行了口服葡萄糖耐量试验，并测定了胰岛素、丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、总胆固醇（TC）和甘油三酯（TG）的水平。京尼平苷、二甲双胍和法舒地尔降低了糖尿病 db/db 小鼠的促炎细胞因子白细胞介素（IL）-1β、IL-6 和肿瘤坏死因子-α的水平。Western blot 分析表明，GE 处理显著逆转了 Rho、ROCK1、ROCK2、p-NF-κBp65 和 p-IκBα 的表达水平。这些发现表明，京尼平苷对糖尿病肝炎症具有保护作用。
