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野生和栽培白 Lupinus albus L. 种子的酚类含量和抗氧化能力。

Phenolic contents and antioxidant capacities of wild and cultivated white lupin (Lupinus albus L.) seeds.

机构信息

Department of Chemical and Physical Properties of Food, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-748 Olsztyn, Poland.

Department of Food Technology, Vocational School of Technical Sciences, Namik Kemal University, 59030 Tekirdağ, Turkey.

出版信息

Food Chem. 2018 Aug 30;258:1-7. doi: 10.1016/j.foodchem.2018.03.041. Epub 2018 Mar 12.

Abstract

The aim of this study was to compare the antioxidant capacities and phenolic compound profiles of wild and cultivated Lupinus albus L. seeds. The total phenolic content (TPC), radical scavenging activity, ferric-reducing antioxidant power (FRAP) and antioxidant activity in an β-carotene-linoleic acid emulsion were determined. Liquid chromatography-mass spectrometry was used to identify phenolics. The TPC of lupin seeds ranged from 4.36 to 7.24 mg gallic acid equivalent/g dry matter (d.m.). The dominant phenolics of all genotypes were two p-coumaric acid derivatives (0.74-1.61 and 0.66-1.63 mg/g d.m.) and apigenin-6,8-di-C-glucoside (1.13-1.31 mg/g d.m.). The results of antioxidant assays of wild lupin extracts were similar to or lower than those of the cultivated variety. FRAP and ABTS scavenging activity were correlated with the contents of the more polar p-coumaric acid derivative and apigenin-6,8-di-C-glucoside. Generally, significant differences between cultivated and wild L. albus seeds were not found in antioxidant capacities and phenolic compound contents.

摘要

本研究旨在比较野生和栽培白 Lupinus albus L. 种子的抗氧化能力和酚类化合物组成。测定了总酚含量(TPC)、自由基清除活性、铁还原抗氧化能力(FRAP)和 β-胡萝卜素-亚油酸乳液中的抗氧化活性。采用液相色谱-质谱联用技术鉴定酚类物质。羽扇豆种子的 TPC 范围为 4.36-7.24mg 没食子酸当量/g 干物质(d.m.)。所有基因型的主要酚类物质均为两种对香豆酸衍生物(0.74-1.61 和 0.66-1.63mg/g d.m.)和芹菜素-6,8-二-C-葡萄糖苷(1.13-1.31mg/g d.m.)。野生羽扇豆提取物的抗氧化测定结果与栽培品种相似或较低。FRAP 和 ABTS 清除活性与更极性的对香豆酸衍生物和芹菜素-6,8-二-C-葡萄糖苷的含量相关。一般来说,在抗氧化能力和酚类化合物含量方面,栽培和野生白 Lupinus albus 种子之间没有发现显著差异。

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