Tang Jia, Saito Takashi
Division of Biochemistry, Department of Oral Biology, School of Dentistry, Health Sciences University of Hokkaido.
Division of Clinical Cariology and Endodontology, Department of Oral Rehabilitation, School of Dentistry, Health Sciences University of Hokkaido.
J Oral Sci. 2018 Jun 20;60(2):253-261. doi: 10.2334/josnusd.17-0286. Epub 2018 Apr 16.
The present study was designed to investigate the effect of laminin-1 (LN-1 or LN-111) on an odontoblast-like cell line, MDPC-23. Wells of non-treated polystyrene plates were coated with various concentrations of LN-1 (0.1, 1, 10, and 100 µg/mL) and left to dry for 2 days. Water-coated surfaces were used as controls. MDPC-23 cell proliferation, differentiation and mineralization were evaluated in terms of the CCK-8 assay, ALP activity, real-time RT-PCR and Alizarin red staining. The data indicated that LN-1 promoted the proliferation of MDPC-23 cells in a concentration-dependent manner. Moreover, it enhanced ALP activity and expression of key odontogenic genes (DMP-1 and DSPP) upon addition of mineralization reagents, leading to significant promotion of calcification by the cells. These results demonstrate that LN-1 acts as an adhesive for odontoblast-like cells, allowing up-regulation of odontogenic genes and accelerating matrix mineralization. In the context of the present study, the optimal LN-1 coating concentration for MDPC-23 cells was suggested to be 100 µg/mL.
本研究旨在探讨层粘连蛋白-1（LN-1或LN-111）对成牙本质细胞样细胞系MDPC-23的影响。将未处理的聚苯乙烯板孔用不同浓度的LN-1（0.1、1、10和100μg/mL）包被，晾干2天。用水包被的表面作为对照。通过CCK-8法、碱性磷酸酶（ALP）活性、实时逆转录聚合酶链反应（RT-PCR）和茜素红染色评估MDPC-23细胞的增殖、分化和矿化。数据表明，LN-1以浓度依赖性方式促进MDPC-23细胞的增殖。此外，添加矿化试剂后，它增强了ALP活性和关键牙源性基因（牙本质基质蛋白-1（DMP-1）和牙本质涎磷蛋白（DSPP））的表达，导致细胞钙化显著增加。这些结果表明，LN-1作为成牙本质细胞样细胞的黏附剂，可上调牙源性基因并加速基质矿化。在本研究中，建议MDPC-23细胞的最佳LN-1包被浓度为100μg/mL。
