Division of Biochemistry, Department of Oral Biology, School of Dentistry, Health Sciences University of Hokkaido, Hokkaido, Japan.
Division of Clinical Cariology and Endodontology, Department of Oral Rehabilitation, School of Dentistry, Health Sciences University of Hokkaido, Hokkaido, Japan.
Biomed Res Int. 2018 May 9;2018:9465383. doi: 10.1155/2018/9465383. eCollection 2018.
The aim for the present study was to evaluate the effects of iMatrix-411 in odontoblast-like cells. To that end, iMatrix-411 was coated to both nontissue culture treated- (Non-PS) and tissue culture treated-polystyrene (TCPS) multiwells. MDPC-23 cells were seeded into noncoated (control) or coated wells. Optimal coating density and cell proliferation were assessed by cell counting kit-8 (CCK-8) at day two, day three, and day five. Osteo/odontogenic differentiation was evaluated by real-time RT-PCR and alkaline phosphatase (ALP) activity at days seven and eight, respectively. Calcific deposition of cells was visualized by alizarin red staining. Data were analyzed with post hoc Tukey HSD test ( < 0.05). Optimal coating density for iMatrix-411 was 8 g/cm. Exposure of MDPC-23 cells to iMatrix-411 in either non-PS or TCPS significantly enhanced proliferative activity. iMatrix-411 elevated ALP activity in both types of culture plates. iMatrix-411 significantly increased the mRNA level of OCN, BSP, OPN, ALP, and DMP-1. Meanwhile, it enhanced the expression of several integrin subunits: ITGA1, ITGA5, ITGAV, ITGB1, and ITGB5. Finally, iMatrix-411 also accelerated the mineralization at day eight in Non-PS. The results indicated iMatrix-411 stimulates proliferation and favours differentiation of odontoblast-like cells.
本研究旨在评估 iMatrix-411 对成牙本质样细胞的作用。为此，将 iMatrix-411 涂覆于未经组织培养处理的（非 PS）和经组织培养处理的聚苯乙烯（TCPS）多孔板上。将 MDPC-23 细胞接种于未涂覆（对照）或涂覆的孔中。通过细胞计数试剂盒-8（CCK-8）在第 2、3 和 5 天评估最佳涂层密度和细胞增殖。通过实时 RT-PCR 和碱性磷酸酶（ALP）活性分别在第 7 和 8 天评估成骨/成牙本质分化。通过茜素红染色可视化细胞的钙化沉积。使用事后 Tukey HSD 检验（<0.05）分析数据。iMatrix-411 的最佳涂层密度为 8 g/cm。MDPC-23 细胞暴露于非 PS 或 TCPS 中的 iMatrix-411 显著增强了增殖活性。iMatrix-411 提高了两种类型培养板中的 ALP 活性。iMatrix-411 显著增加了 OCN、BSP、OPN、ALP 和 DMP-1 的 mRNA 水平。同时，它还增强了几个整合素亚基的表达：ITGA1、ITGA5、ITGAV、ITGB1 和 ITGB5。最后，iMatrix-411 还加速了非 PS 中第 8 天的矿化。结果表明，iMatrix-411 刺激成牙本质样细胞的增殖和分化。
