基于半巢式聚合酶链反应检测伊朗西北部小反刍动物中的 spp.。
Semi-nested polymerase chain reaction-based detection of spp. in small ruminants from Northwest of Iran.
作者信息
Bazmani Ahad, Abolhooshyar Amir, Imani-Baran Abbas, Akbari Hamid
机构信息
Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran.
出版信息
Vet World. 2018 Mar;11(3):268-273. doi: 10.14202/vetworld.2018.268-273. Epub 2018 Mar 3.
AIM
The present study aimed to detect and in the blood samples of sheep and goats from Northwest of Iran by the semi-nested polymerase chain reaction (PCR) technique.
MATERIALS AND METHODS
A total of 166 whole blood samples (including 123 sheep and 43 goats) were collected. In the first stage, the PCR was performed to amplify a piece of 18S rRNA gene of and genera. Then, semi-nested PCR was carried out on all PCR products to differentiate and .
RESULTS
The PCR indicated that totally, 19 (11.44%) out of 166 samples were positive for or spp. The semi-nested PCR showed that 38 samples (22.89%) were positive only for . No significant association was found between the infection rate of and age, gender and species of animals.
CONCLUSION
In the present study, there was no evidence for infection in small ruminants from Northwest of Iran. Therefore, was the main causative agent of ovine Babesiosis in this region.
目的
本研究旨在通过半巢式聚合酶链反应（PCR）技术检测伊朗西北部绵羊和山羊血液样本中的 和 。
材料与方法
共采集了166份全血样本（包括123只绵羊和43只山羊）。在第一阶段，进行PCR以扩增 属和 属的一段18S rRNA基因。然后，对所有PCR产物进行半巢式PCR以区分 和 。
结果
PCR结果表明，166份样本中共有19份（11.44%）对 或 属呈阳性。半巢式PCR显示，38份样本（22.89%）仅对 呈阳性。未发现 的感染率与动物的年龄、性别和种类之间存在显著关联。
结论
在本研究中，没有证据表明伊朗西北部的小反刍动物感染了 。因此， 是该地区绵羊巴贝斯虫病的主要病原体。
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