Naderi Arash, Nayebzadeh Hassan, Gholami Shirzad
Department of Parasitology, Science and Research BranchIslamic Azad University, Boroujerd, Iran.
Department of Parasitology, Faculty of Veterinary MedicineLorestan University, Khorramabad, Iran.
J Parasit Dis. 2017 Sep;41(3):837-842. doi: 10.1007/s12639-017-0899-1. Epub 2017 Feb 28.
is a lethal protozoan disease, responsible for the loss of livestock in Iran and in the world. The purpose of the current study was to detect and identify spp. infection using microscopic and molecular methods in human, sheep and goats in Kuhdasht region, in the Lorestan Province, west of Iran. During 2013, a total of 384 blood smear samples were collected from 51 goats, 306 sheep suspected of infection and 27 humans from Kuhdasht region. The blood samples were fixed, stained and under light microscopic examined. DNA samples were extracted and amplified by polymerase chain reaction of 18S-rRNA gene. PCR and the semi-nested PCR were performed to identify to and to differentiate genus of and spp. The results of microscopic examination indicated that a total of 47 (12.2%) samples were positive for spp. infection: 38 (9.9%) belonging to sheep and 9 to goats (2.3%). No was observed in human samples. The PCR showed a band size of 389 bp, of spp. and the semi-nested PCR detected with a band size of 186 bp. By molecular method, 16 (4.2%) sheep and 2 (0.5%) goat blood samples were infected by . Totally, 18 samples (4.7%) were observed to have a, while no infection was found in human. Thus, the results of our study have shown sheep and goats could be vulnerable to spp., especially in Lorestan Province, Iran. Therefore, studies on the status of the animal especially are recommended.
是一种致命的原生动物疾病，在伊朗乃至全世界都导致了牲畜的损失。本研究的目的是利用显微镜和分子方法，在伊朗西部洛雷斯坦省库赫达什特地区的人类、绵羊和山羊中检测和鉴定 属物种感染情况。2013年期间，共从库赫达什特地区的51只山羊、306只疑似感染的绵羊和27名人类身上采集了384份血涂片样本。血样经固定、染色后进行光学显微镜检查。提取DNA样本并通过18S - rRNA基因的聚合酶链反应进行扩增。进行PCR和半巢式PCR以鉴定 并区分 属和 属物种。显微镜检查结果表明，共有47份（12.2%）样本 属物种感染呈阳性：38份（9.9%）属于绵羊，9份属于山羊（2.3%）。在人类样本中未观察到 。PCR显示 属物种的条带大小为389 bp，半巢式PCR检测到 的条带大小为186 bp。通过分子方法，16份（4.2%）绵羊和2份（0.5%）山羊血样感染了 。总共观察到18份样本（4.7%）感染了 ，而在人类中未发现感染。因此，我们的研究结果表明绵羊和山羊可能易受 属物种感染，尤其是在伊朗洛雷斯坦省。因此，建议对动物 尤其是 的状况进行研究。
