利用釉原蛋白基因从印度儿童额外牙进行性别鉴定。
Sex determination from mesiodens of Indian children by amelogenin gene.
作者信息
Srivastava Mohit, Tripathi Swati, Astekar Madhusudan, Singal Deepa, Srivastava Aditi, Vashisth Pallavi
机构信息
Department of Pedodontics and Preventive Dentistry, Karnavathi School of Dentistry, Gandhinagar, Gujarat, India.
Department of Pedodontics and Preventive Dentistry, Institute of Dental Sciences, Mahatma Jyotiba Phule Rohilkhand University, Bareilly, Uttar Pradesh, India.
出版信息
J Forensic Dent Sci. 2017 Sep-Dec;9(3):125-129. doi: 10.4103/jfo.jfds_72_16.
CONTEXT
The identification of sex is the first and the foremost step in forensic science. Teeth consist of enamel which is the hardest tissue available in the body, protector of DNA presents in pulp tissue at the time of exposure of tooth to adverse conditions. Teeth can be stated as a sealed box of mystery as it contains various human and bacterial DNA for molecular utilization.
AIM AND OBJECTIVE
The aim is to determine sex from mesiodens on the basis of gene identification by the polymerase chain reaction (PCR).
SETTINGS AND DESIGN
Total number of sample was 8 human-extracted mesiodens. DNA was isolated and was subjected to PCR analysis with use of predesigned primers for amelogenin (AMEL) X and AMEL Y genes.
RESULTS
Isolation of genomic and mitochondrial DNA from mesiodens was successful in six samples (75%). In samples, quantity of DNA present was also calculated.
CONCLUSION
Mesiodens are a good source of DNA and are a very useful tool in identification of sex using PCR analysis which was simple and effective. Hence, the procedure presented in the present study can be applied for extraction of DNA and identification of sex for forensic purpose.
背景
性别鉴定是法医学的首要步骤。牙齿由牙釉质构成，牙釉质是人体中最坚硬的组织，在牙齿暴露于不利条件时，它能保护牙髓组织中的DNA。牙齿可被视为一个神秘的密封盒，因为它包含各种用于分子利用的人类和细菌DNA。
目的
旨在通过聚合酶链反应（PCR）进行基因鉴定，从额外牙中确定性别。
设置与设计
样本总数为8颗从人体提取的额外牙。分离DNA，并使用针对牙釉蛋白（AMEL）X和AMEL Y基因的预先设计引物进行PCR分析。
结果
在六个样本（75%）中成功从额外牙分离出基因组和线粒体DNA。还计算了样本中存在的DNA数量。
结论
额外牙是DNA的良好来源，是使用简单有效的PCR分析进行性别鉴定的非常有用的工具。因此，本研究中提出的程序可用于法医目的的DNA提取和性别鉴定。
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