Maiborodin I V, Morozov V V, Anikeev A A, Figurenko N F, Maslov R V, Matveeva V A, Maiborodina V I
Center of New Medical Technologies, Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
Laboratory of Ultrastructural Bases of Pathology, Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia.
Bull Exp Biol Med. 2018 Apr;164(6):784-789. doi: 10.1007/s10517-018-4080-3. Epub 2018 Apr 16.
Hemorrhagic changes after subcutaneous injection of autologous bone marrow multipotent mesenchymal cells with transfected GFP gene and additionally stained cell membranes to WAG rats in the projection of ligated femoral vein were studied by fluorescent microscopy. Hemorrhages in tissues with experimental acute local venous occlusion were caused by a combination of venous hypertension with inflammation around the foreign body - the ligature used for ligation of the vein. Fibrin found in tissues together with erythrocytes in the hemorrhages could stimulate the formation of granulations and new vessels instead of damaged or thrombosed ones. Multipotent mesenchymal stromal cells and their detritus getting into the regional lymph nodes initiated immune reactions morphologically confirmed by stubborn hypertrophy and hyperplasia of the lymphoid nodules, hemorrhages, and manifest diapedesis of erythrocytes to the organ parenchyma and sinus system.
通过荧光显微镜研究了在结扎股静脉投影处向WAG大鼠皮下注射转染绿色荧光蛋白（GFP）基因的自体骨髓多能间充质细胞并额外对细胞膜进行染色后的出血变化。实验性急性局部静脉闭塞组织中的出血是由静脉高压与异物（用于结扎静脉的结扎线）周围的炎症共同引起的。在出血组织中发现的与红细胞一起的纤维蛋白可以刺激肉芽和新血管的形成，以替代受损或血栓形成的血管。多能间充质基质细胞及其碎屑进入局部淋巴结引发免疫反应，形态学上表现为淋巴小结顽固的肥大和增生、出血以及红细胞明显渗出到器官实质和窦系统。
