[精子发生过程中小鼠睾丸中Daxx基因的表达特征]
[Expression characteristics of the Daxx gene in the mouse testis during spermatogenesis].
作者信息
Zhang Zeng, Deng Qiong, Wu Yong, Huang Xin-Bo, Yao Lu, Jiang Zhi-Mao, Gui Yao-Ting
机构信息
Graduate School, Anhui Medical University, Hefei, Anhui 230032, China.
Key Laboratory of Male Reproductive Medicine and Genetics, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, China.
出版信息
Zhonghua Nan Ke Xue. 2017 Feb;23(2):103-109.
OBJECTIVE
To investigate the expression characteristic of the Daxx gene in the mouse testis and its role in spermatogenesis.
METHODS
Real-time PCR, Western blot and immunofluorescence were used in examining the expression characteristics of DAXX in the testis tissue from wild-type, Sertoli cell-specific androgen receptor knockout (SCARKO) and androgen receptor knockout (ARKO) mice at different postnatal weeks .
RESULTS
The Daxx gene was highly expressed in the testis tissue and mainly in the nuclei of the wild-type mice at 4 postnatal weeks. Compared with the wild-type, the ARKO mice showed a markedly decreased expression of DAXX (0.299±0.026), which displayed a polar distribution in the spermatogenic cells (0.853±0.058) and exhibited no significant difference in the SCARKO mice (1.000±0.015).
CONCLUSIONS
The Daxx gene expression is the highest in the middle-stage development of the mouse testis, significantly decreased in ARKO mice as compared with the wild-type, and its location influenced by specific AR knockout in Sertoli cells. DAXX may be involved in the regulation of spermatogenesis in mice.
目的
研究Daxx基因在小鼠睾丸中的表达特征及其在精子发生中的作用。
方法
采用实时荧光定量PCR、蛋白质免疫印迹法和免疫荧光法检测野生型、支持细胞特异性雄激素受体敲除（SCARKO）和雄激素受体敲除（ARKO）小鼠在不同出生后周龄时睾丸组织中DAXX的表达特征。
结果
Daxx基因在睾丸组织中高表达，在出生后4周的野生型小鼠中主要定位于细胞核。与野生型相比，ARKO小鼠中DAXX的表达显著降低（0.299±0.026），在生精细胞中呈极性分布（0.853±0.058），而在SCARKO小鼠中无显著差异（1.000±0.015）。
结论
Daxx基因在小鼠睾丸发育中期表达最高，与野生型相比，ARKO小鼠中表达显著降低，其定位受支持细胞中特异性雄激素受体敲除的影响。DAXX可能参与小鼠精子发生的调控。
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