UVA照射的人视网膜色素上皮细胞中渗透溶质牛磺酸的诱导作用
Osmolyte taurine induction in UVA exposed human retinal pigment epithelial cells.
作者信息
Dayang Wu, Dongbo Pang
机构信息
a Department of Ophthalmology , The First Affiliated Hospital of Jinzhou Medical University , Jinzhou , P.R. China.
出版信息
Cutan Ocul Toxicol. 2018 Dec;37(4):338-343. doi: 10.1080/15569527.2018.1465078. Epub 2018 May 3.
AIM
To explore the osmolytes expression in ultraviolet (UVA) stressed human retinal pigment epithelial cells.
METHODS
Osmolyte transporters and vascular endothelial growth factor (VEGF) messenger RNA (mRNA) were determined by real time polymerase chain reaction (PCR). Osmolyte uptake was measured by radioimmunoassay. VEGF concentrations were determined by immunoassay and enzyme-linked immunosorbent assay (ELISA). Osmolyte taurine transporter (TAUT) were silenced by siRNA technology.
RESULTS
Hypertonicity accelerated osmolyte betaine uptake, myoinositol uptake, and taurine uptake, compared to normotonic stress. UVA irradiation also accelerated osmolyte transporters expression and osmolytes uptake. Especially, osmolyte taurine remarkably inhibited VEGF release induced by UVA irradiation. VEGF in the UVA stressed retinal pigment epithelial cell supernatant was accumulated slow after taurine preincubation. VEGF expression increased significantly in UVA-stressed cells after TAUT silencing. Moreover, taurine reduced the VEGF level in human ocular aqueous humor.
CONCLUSION
The inhibition of VEGF by osmolyte taurine plays the crucial role in retina adaption to UVA irradiation.
目的
探讨渗透压调节剂在紫外线（UVA）应激的人视网膜色素上皮细胞中的表达情况。
方法
采用实时聚合酶链反应（PCR）检测渗透压调节剂转运体和血管内皮生长因子（VEGF）信使核糖核酸（mRNA）。通过放射免疫分析法测定渗透压调节剂摄取量。采用免疫分析法和酶联免疫吸附测定法（ELISA）测定VEGF浓度。利用小干扰RNA（siRNA）技术使渗透压调节剂牛磺酸转运体（TAUT）沉默。
结果
与等渗应激相比，高渗状态加速了渗透压调节剂甜菜碱摄取、肌醇摄取和牛磺酸摄取。UVA照射也加速了渗透压调节剂转运体的表达和渗透压调节剂摄取。特别是，渗透压调节剂牛磺酸显著抑制UVA照射诱导的VEGF释放。牛磺酸预孵育后，UVA应激的视网膜色素上皮细胞上清液中的VEGF积累缓慢。TAUT沉默后，UVA应激细胞中的VEGF表达显著增加。此外，牛磺酸降低了人眼房水中的VEGF水平。
结论
渗透压调节剂牛磺酸对VEGF的抑制作用在视网膜适应UVA照射中起关键作用。
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