School of Food and Biological Engineering, Hubei University of Technology, Wuhan, Hubei 430068, P.R. China.
Mol Med Rep. 2018 Jun;17(6):8403-8408. doi: 10.3892/mmr.2018.8879. Epub 2018 Apr 12.
The influence of lecithin from egg yolk (LE) on calcium carbonate (CaCO3) biomineralization was investigated. In the present study, spherical CaCO3 particles were synthesized via coprecipitation in the presence of LE. LE multilamellar liposomes were first tuned by sonication to provide better control over the nucleation of CaCO3. Subsequently, monodisperse microspheres ~2 µm in size were generated by controlling the aggregation and growth of CaCO3 under appropriate concentrations of LE. In contrast to unstable vaterite, the microspheres generated in aqueous solution remained stable for at least 10 days without transforming into calcite, due to the strong interaction between the LE and calcium ions. The microspheres as drug carriers of doxorubicin (DOX) were assessed and were observed to have a good encapsulation efficiency, sustained drug release without a burst release and notable pH sensitivity. In addition, in vivo tumor inhibition examination demonstrated that DOX‑loaded CaCO3 microspheres formulation had more superior efficacy to significantly restrain tumor growth. These novel LE/CaCO3 hybrids may provide novel options for various biomedical applications.
研究了蛋黄卵磷脂 (LE) 对碳酸钙 (CaCO3) 生物矿化的影响。本研究通过共沉淀在 LE 的存在下合成了球形 CaCO3 颗粒。通过超声处理首先调整 LE 的多层脂质体，以更好地控制 CaCO3 的成核。随后，通过控制 LE 的适当浓度下 CaCO3 的聚集和生长，生成了尺寸约为 2 µm 的单分散微球。与不稳定的球霰石不同，由于 LE 和钙离子之间的强相互作用，在水溶液中生成的微球至少在 10 天内保持稳定，而不会转化为方解石。作为阿霉素 (DOX) 的药物载体的微球进行了评估，观察到其具有良好的包封效率、无突释的持续药物释放和显著的 pH 敏感性。此外，体内肿瘤抑制实验表明，载 DOX 的 CaCO3 微球制剂具有更优异的疗效，能够显著抑制肿瘤生长。这些新型 LE/CaCO3 杂化物可为各种生物医学应用提供新的选择。
