Department of Biotechnology, University of Rijeka, Rijeka, Croatia.
Department of Chemistry, J. J. Strossmayer University Osijek, Osijek, Croatia.
Electrophoresis. 2018 Jul;39(13):1527-1542. doi: 10.1002/elps.201800029. Epub 2018 May 8.
Representative sampling and adequate sample preparation are key factors for successful performance of further steps in foodomic analyses, as well as for correct data interpretation. Incorrect sampling and improper sample preparation can be sources of severe bias in foodomic analyses. It is well known that both wrong sampling and sample treatment cannot be corrected anymore. These, in the past frequently neglected facts, are now taken into consideration, and the progress in sampling and sample preparation in foodomics is reviewed here. We report the use of highly sophisticated instruments for both high-performance and high-throughput analyses, as well as miniaturization and the use of laboratory robotics in metabolomics, proteomics, peptidomics, and genomics.
代表性采样和充分的样品制备是成功进行食品组学分析的关键因素，也是正确数据解释的关键。不正确的采样和不适当的样品制备可能是食品组学分析中严重偏差的来源。众所周知，错误的采样和样品处理都无法再进行纠正。这些过去经常被忽视的事实现在都被考虑在内，本文回顾了食品组学中采样和样品制备的进展。我们报告了使用高度复杂的仪器进行高效和高通量分析，以及在代谢组学、蛋白质组学、肽组学和基因组学中进行的微型化和实验室机器人的使用。
