Ishiura S, Koizumi H, Tsukahara T, Sugita H
National Institute of Neuroscience, NCNP, Tokyo.
J Biochem. 1988 Jan;103(1):11-3. doi: 10.1093/oxfordjournals.jbchem.a122214.
A cytolytic protein (perforin) was rapidly purified from a cell line of mouse cytotoxic T-lymphocytes (CTL) by DEAE-cellulose, heparin-Sepharose, and phenyl-Sepharose chromatographies. The purified perforin was activated by heparin, the half maximal concentration being 3-10 ng/ml, depending on the calcium concentration. Other acid mucopolysaccharides, such as chondroitin sulfates A and C, keratan polysulfate, and heparin sulfate, also enhanced the lysis of erythrocytes by perforin, but the concentrations required for activation were more than 100-fold higher than that of heparin. Chondroitin, hyaluronic acid, and keratan sulfate, however, had no effect on the perforin activity. It was suggested that heparin potentiates the lytic activity of perforin and acid mucopolysaccharides may actually be involved in target cell lysis by CTL.
通过DEAE - 纤维素、肝素 - 琼脂糖和苯基 - 琼脂糖色谱法，从小鼠细胞毒性T淋巴细胞（CTL）细胞系中快速纯化出一种溶细胞蛋白（穿孔素）。纯化的穿孔素可被肝素激活，其半数最大激活浓度为3 - 10 ng/ml，具体取决于钙浓度。其他酸性粘多糖，如硫酸软骨素A和C、多硫酸角质素和硫酸肝素，也能增强穿孔素对红细胞的裂解作用，但激活所需浓度比肝素高100倍以上。然而，软骨素、透明质酸和硫酸角质素对穿孔素活性没有影响。有人提出，肝素可增强穿孔素的裂解活性，酸性粘多糖可能实际上参与了CTL对靶细胞的裂解。
