Mosole Simone, Zampieri Sandra, Furlan Sandra, Carraro Ugo, Löefler Stefan, Kern Helmut, Volpe Pompeo, Nori Alessandra
University of Padova, Italy.
Ludwig Boltzmann Institute of Electrical Stimulation and Physical Rehabilitation, Vienna, Austria.
Gerontol Geriatr Med. 2018 Apr 10;4:2333721418768998. doi: 10.1177/2333721418768998. eCollection 2018 Jan-Dec.
Physical activity plays an important role in preventing muscle atrophy and chronic diseases in adults and in the elderly. Calcium (Ca) cycling and activation of specific molecular pathways are essential in contraction-induced muscle adaptation. This study attains human muscle sections and total homogenates prepared from biopsies obtained before (control) and after 9 weeks of training by electrical stimulation (ES) on a group of volunteers. The aim of the study was to investigate about the molecular mechanisms that support functional muscle improvement by ES. Evidences of kinase/phosphatase pathways activation after ES were obtained. Moreover, expression of Sarcalumenin, Calsequestrin and sarco/endoplasmic reticulum Ca-ATPase (Serca) isoforms was regulated by training. In conclusion, this work shows that neuromuscular ES applied to vastus lateralis muscle of sedentary seniors combines fiber remodeling with activation of Ca-Calmodulin molecular pathways and modulation of key Ca-handling proteins.
体育活动在预防成年人和老年人的肌肉萎缩及慢性疾病方面发挥着重要作用。钙（Ca）循环和特定分子途径的激活在收缩诱导的肌肉适应中至关重要。本研究获取了一组志愿者在接受9周电刺激（ES）训练前（对照）和训练后的活检样本制备的人体肌肉切片和总匀浆。该研究的目的是探究支持电刺激改善肌肉功能的分子机制。获得了电刺激后激酶/磷酸酶途径激活的证据。此外，训练可调节肌质网蛋白、肌钙蛋白和肌浆/内质网Ca-ATP酶（Serca）亚型的表达。总之，这项工作表明，应用于久坐老年人股外侧肌的神经肌肉电刺激将纤维重塑与Ca-钙调蛋白分子途径的激活以及关键钙处理蛋白的调节相结合。
