分泌组小鼠为描绘组织特异性体内分泌提供了遗传平台。
The secretome mouse provides a genetic platform to delineate tissue-specific in vivo secretion.
机构信息
Aging Institute, University of Pittsburgh School of Medicine/UPMC, Pittsburgh, PA 15219.
Department of Medicine, Division of Cardiology, University of Pittsburgh/UPMC, Pittsburgh, PA 15219.
出版信息
Proc Natl Acad Sci U S A. 2021 Jan 19;118(3). doi: 10.1073/pnas.2005134118.
Abstract
At present, it remains difficult to deconvolute serum in order to identify the cell or tissue origin of a given circulating protein. Here, by exploiting the properties of proximity biotinylation, we describe a mouse model that enables the elucidation of the in vivo tissue-specific secretome. As an example, we demonstrate how we can readily identify in vivo endothelial-specific secretion as well as how this model allows for the characterization of muscle-derived serum proteins that either increase or decrease with exercise. This genetic platform should, therefore, be of wide utility in understanding normal and disease physiology and for the rational design of tissue-specific disease biomarkers.
摘要
目前，要对血清进行去卷积以鉴定特定循环蛋白的细胞或组织来源仍然具有挑战性。在这里，我们利用邻近生物素化的特性，描述了一种能够阐明体内组织特异性分泌组的小鼠模型。例如，我们展示了如何能够轻易地鉴定体内血管内皮特异性分泌，以及该模型如何允许对运动时增加或减少的肌肉来源的血清蛋白进行特征分析。因此，该遗传平台应该在理解正常和疾病生理学以及合理设计组织特异性疾病生物标志物方面具有广泛的应用价值。
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