Lamy B, Hervé P, Tissot C, Peters A
Centre Régional de Transfusion Sanguine, Besançon, France.
Pathol Biol (Paris). 1988 Jan;36(1):29-36.
Flow cytometry is a biological analysis technics whose applications may be used for minimal residual disease evaluation. From these applications we retained the DNA content measurement (with DNA index), surface markers analysis, and flow karyotyping. The threshold cell aneuploidy detection depend of the DNA index of leukemia cell population. The establishment of a relation between cell surface markers and malignant character of the cell is difficult to obtain. Study of CALLA antigen in leukemic patients have shown variations of antigenic density with different categories of studied patients. Malignant cells studied at the diagnosis or at the relapse of leukemia have a mean antigenic density higher than normal cells. This fact could be used to evaluate minimal residual disease as shown with recent clinical observations.
流式细胞术是一种生物学分析技术，其应用可用于微小残留病评估。从这些应用中，我们保留了DNA含量测量（通过DNA指数）、表面标志物分析和流式核型分析。阈值细胞非整倍体检测取决于白血病细胞群体的DNA指数。很难确定细胞表面标志物与细胞恶性特征之间的关系。对白血病患者CALLA抗原的研究表明，不同研究患者类别中抗原密度存在差异。在白血病诊断或复发时研究的恶性细胞的平均抗原密度高于正常细胞。正如最近的临床观察所示，这一事实可用于评估微小残留病。
