Sadove R, Ladaga L, Magee W P
Department of Surgery, University of Kentucky Medical Center, Lexington.
Plast Reconstr Surg. 1988 May;81(5):655-61. doi: 10.1097/00006534-198805000-00001.
In an effort to prove the etiology of the cleft lip nasal deformity, 23 patients with unilateral cleft lip underwent biopsy through the midportion of the columella from mucosa to mucosa. This tissue "sandwich" contained an internal control of cleft and noncleft medial crus cartilage. With the use of special stains and examination under the microscope at low, medium, and high powers, sections were evaluated on the basis of presence of abnormal chondrocytes, number of binucleate chondrocytes, number of nucleated chondrocytes, number of lacunae, perichondrial thickness, and cartilage thickness. In each specimen examined, there were no significant differences between cleft and noncleft sides, proving histologically the previous subjective observation that the deformity is extrinsic, due to distortion of the lower lateral cartilage by abnormal vectors of force.
为了证实唇裂鼻畸形的病因，23例单侧唇裂患者经鼻小柱中部从黏膜到黏膜进行活检。这种组织“三明治”包含腭裂和非腭裂内侧脚软骨的内部对照。通过使用特殊染色并在低倍、中倍和高倍显微镜下检查，根据异常软骨细胞的存在、双核软骨细胞的数量、有核软骨细胞的数量、腔隙的数量、软骨膜厚度和软骨厚度对切片进行评估。在每个检查的标本中，腭裂侧和非腭裂侧之间没有显著差异，从组织学上证明了先前的主观观察结果，即该畸形是外在的，是由于异常力向量导致下外侧软骨扭曲所致。
