Brooks S P, Storey K B
Institute of Biochemistry, Carleton University Ottawa, Ontario, Canada.
FEBS Lett. 1988 May 9;232(1):214-6. doi: 10.1016/0014-5793(88)80419-8.
An examination of the kinetic parameters of phosphofructokinase, pyruvate kinase and glycogen phosphorylase, and the cellular concentration of fructose 2,6-bisphosphate during anoxia in the turtle Pseudemys scripta showed that the total activity of glycogen phosphorylase, and the phosphofructokinase inhibition constants for citrate and ATP were decreased in anoxic turtle brain. These results suggest that the ability of turtle brain to survive extended periods of anoxia is the result of metabolic rate depression regulated, at the molecular level, by enzyme inactivation through anoxia-induced covalent modification.
对伪彩龟（Pseudemys scripta）在缺氧期间磷酸果糖激酶、丙酮酸激酶和糖原磷酸化酶的动力学参数以及细胞内果糖-2,6-二磷酸浓度的检查表明，缺氧的龟脑内糖原磷酸化酶的总活性以及柠檬酸和ATP对磷酸果糖激酶的抑制常数均降低。这些结果表明，龟脑在长时间缺氧状态下存活的能力是代谢率降低的结果，在分子水平上，这是由缺氧诱导的共价修饰导致的酶失活所调节的。
